您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification and Fine Mapping of Gummy Stem Blight Resistance Gene Gsb-7(t) in Melon

文献类型： 外文期刊

作者： Ma, Jian 1 ; Li, Congcong 3 ; Tian, Jiaxing 1 ; Qiu, Yanhong 1 ; Geng, Lihua 1 ; Wang, Jianshe 1 ;

作者机构： 1.Minist Agr & Rural Affairs, Key Lab Biol & Genet Improvement Hort Crops North, Beijing 100097, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr BVRC, Beijing 100097, Peoples R China

3.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

关键词： gene mapping; Gsb-7(t); gummy stem blight; melon; resistance gene

期刊名称：PHYTOPATHOLOGY （ 影响因子：3.2； 五年影响因子：3.9 ）

ISSN： 0031-949X

年卷期： 2023 年 113 卷 5 期

页码：

收录情况： SCI

摘要： Gummy stem blight (GSB), caused by Didymella bryoniae, is a devastating fungal disease of melon worldwide. Breeding GSB-resistant cultivars with host resistance genes is considered the most economic and effective strategy to control this disease. In this study, 260 melon germplasm resources were screened for resistance to GSB, and an inbred line, H55R, that exhibited immunity to GSB was identified. To further understand the resistance mechanism of H55R against GSB, an F-2 population was obtained from a cross between the GSB-susceptible line A15 and H55R, and genetic analysis indicated that the GSB resistance in H55R was controlled by a single dominant gene, tentatively named Gsb-7(t). The Gsb-7(t) gene was finally delimited to a 140-kb interval on chromosome 7 using bulked segregant analysis and chromosome walking strategies. Ten putative genes were annotated in this region that contains a wall-associated receptor kinase (WAK) gene MELO3C010403. The MELO3C010403 gene contains two alternative transcripts, MELO3C010403-T1 and MELO3C010403-T2, with five and seven nonsynonymous mutation sites, respectively. Gene expression analysis showed that expression of MELO3C010403-T2 but not MELO3C010403-T1 was significantly induced by D. bryoniae at 24 h postinoculation, indicating that the MELO3C010403-T2 transcript of MELO3C010403 was the most likely candidate gene of Gsb-7(t). Our results offer new genetic resources and will be helpful for the development of GSB-resistant melon cultivars in the future.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台