Diverse triterpene skeletons are derived from the expansion and divergent evolution of 2,3-oxidosqualene cyclases in plants
文献类型： 外文期刊
作者： Wang, Jing 1 ; Guo, Yanhong 1 ; Yin, Xue 1 ; Wang, Xiaoning 4 ; Qi, Xiaoquan 5 ; Xue, Zheyong 1 ;
作者机构： 1.Northeast Forestry Univ, Minist Educ, Key Lab Saline Alkali Vegetat Ecol Restorat, Harbin, Peoples R China
2.Northeast Forestry Univ, Heilongjiang Key Lab Plant Bioact Subst Biosynthe, Harbin, Peoples R China
3.Beijing Acad Agr & Forestry Sci, Inst Forestry & Pomol, Beijing, Peoples R China
4.Shandong Univ, Sch Pharmaceut Sci, Minist Educ, Dept Nat Prod Chem,Key Lab Chem Biol, Jinan, Peoples R China
5.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China
关键词： Triterpene; oxidosqualene cyclase; functional divergence; catalytic mechanism; biosynthesis
期刊名称：CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：8.25； 五年影响因子：9.0 ）
ISSN： 1040-9238
年卷期：
页码：
收录情况： SCI
摘要： Triterpenoids are one of the largest groups of secondary metabolites and exhibit diverse structures, which are derived from C30 skeletons that are biosynthesized via the isoprenoid pathway by cyclization of 2,3-oxidosqualene. Triterpenoids have a wide range of biological activities, and are used in functional foods, drugs, and as industrial materials. Due to the low content levels in their native plants and limited feasibility and efficiency of chemical synthesis, heterologous biosynthesis of triterpenoids is the most promising strategy. Herein, we classified 121 triterpene alcohols/ketones according to their conformation and ring numbers, among which 51 skeletons have been experimentally characterized as the products of oxidosqualene cyclases (OSCs). Interestingly, 24 skeletons that have not been reported from nature source were generated by OSCs in heterologous expression. Comprehensive evolutionary analysis of the identified 152 OSCs from 75 species in 25 plant orders show that several pentacyclic triterpene synthases repeatedly originated in multiple plant lineages. Comparative analysis of OSC catalytic reaction revealed that stabilization of intermediate cations, steric hindrance, and conformation of active center amino acid residues are primary factors affecting triterpene formation. Optimization of OSC could be achieved by changing of side-chain orientations of key residues. Recently, methods, such as rationally design of pathways, regulation of metabolic flow, compartmentalization engineering, etc., were introduced in improving chassis for the biosynthesis of triterpenoids. We expect that extensive study of natural variation of large number of OSCs and catalytical mechanism will provide basis for production of high level of triterpenoids by application of synthetic biology strategies.
- 相关文献
作者其他论文 更多>>
-
EasyMetagenome: A user-friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research
作者：Bai, Defeng;Xun, Jiani;Ma, Chuang;Luo, Hao;Yang, Haifei;Hou, Huiyu;Lv, Hujie;Wan, Xiulin;Wang, Yao;Yousuf, Salsabeel;Zeng, Meiyin;Zhang, Tianyuan;Gao, Yunyun;Liu, Yong-Xin;Chen, Tong;Ma, Chuang;Yang, Haifei;Cao, Chen;Cao, Xiaofeng;Cui, Jianzhou;Deng, Yuan-Ping;Deng, Zhaochao;Yu, Hao;Zhang, Chunfang;Dong, Wenxin;Dong, Wenxue;Du, Juan;Fang, Qunkai;Fang, Wei;Fang, Yue;Luan, Yaning;Fu, Fangtian;Fu, Min;Fu, Yi-Tian;Gao, He;Ge, Jingping;Guo, Yuhao;Gong, Qinglong;Lou, Wenbo;Gu, Lunda;Yang, Li;Guo, Peng;Hai, Tang;Liu, Hao;He, Jieqiang;He, Zi-Yang;Huang, Can;Ji, Shuai;Jiang, ChangHai;Jiang, Gui-Lai;Jiang, Lingjuan;Jin, Ling N.;Li, Changchao;Kan, Yuhe;Kang, Da;Kou, Jin;Lam, Ka-Lung;Li, Chong;Li, Fuyi;Li, Liwei;Li, Miao;Li, Xin;Li, Ye;Li, Zheng-Tao;Zhu, Chengshuai;Liang, Jing;Mo, Jiayuan;Lin, Yongxin;Liu, Changzhen;Liu, Danni;Zhang, Jing;Chen, Shifu;Liu, Fengqin;Liu, Jia;Liu, Tianrui;Liu, Tingting;Wang, Xinlong;Liu, Xinyuan;Luo, Yuanyuan;Liu, Yaqun;Liu, Bangyan;Liu, Minghao;Lv, Hujie;Ma, Tengfei;Mai, Zongjiong;Niu, Dongze;Pan, Zhuo;Qi, Heyuan;Shi, Zhanyao;Song, Chunjiao;Sun, Fuxiang;Sun, Yan;Tian, Sihui;Wang, Guoliang;Wang, Hongyang;Wang, Hongyu;Wang, Huanhuan;Wang, Jing;Wang, Jun;Wang, Kang;Wang, Leli;Yao, Xiaofang;Wang, Shao-kun;Xiao, Zufei;Xing, Huichun;Xu, Yifan;Yang, Song;Yan, Shu-yan;Zhang, Yi-Bo;Yang, Yuanming;Lei, Yu;Yuan, Zhengrong;Zhang, Chunge;Zhang, Huimin;Zhang, Na;Zhang, Yupeng;Zhang, Zheng;Zhou, Mingda;Zhou, Yuanping;Zhu, Zhihao;Zhu, Lin;Zhu, Yue;Zou, Hongqin;Zuo, Anna;Dong, Wenxuan;Wen, Tao;Chen, Shifu;Chen, Shifu;Li, Guoliang
关键词：metagenome; microbiome; microbiota; pipeline; visualization
-
Calycosin, a Bioactive Isoflavone, Ameliorates Oxidative Stress and Inflammation in Lipopolysaccharide-Induced Intestinal Cell Damage Model via the Nrf2 and NF-κB Signaling Pathways
作者：Li, Lu;Che, Yuyan;Zhu, Longlong;Liu, Zeqing;Song, Yanchen;Chen, Meixia;Wang, Jing;Li, Lu;Che, Yuyan;Song, Yanchen;Zhu, Longlong;Liu, Zeqing;Wu, Qiong;Chen, Meixia;Wang, Jing
关键词：calycosin; cell damage; inflammation; LPS; NF-kappa B pathway; Nrf2 pathway; oxidative stress
-
Chromosome-level genome assembly of tetraploid Chinese cherry (Prunus pseudocerasus)
作者：Zhang, Wei;Wang, Xuncheng;Peng, Junbo;Xing, Qikai;Yan, Jiye;Wang, Jing;Duan, Xuwei;Zhang, Xiaoming;Zhang, Kaichun
关键词：
-
New insights into the cold tolerance of upland switchgrass by integrating a haplotype-resolved genome and multi-omics analysis
作者：Wu, Bingchao;Luo, Dan;Yan, Haidong;Ma, Xixi;Zhu, Jie;Jia, Jiyuan;Sun, Min;Xie, Zheni;Wang, Xiaoshan;Huang, Linkai;Yue, Yuesen;He, Min;Zhao, Bingyu;Xu, Bin;Wang, Jing;Sun, Min
关键词：Upland switchgrass; Haplotype-resolved genomes; Allele-specific expression genes; Cold tolerance
-
Identification and characteristic analysis of PavUGT48 as a novel UDP-glycosyltransferase with dual functions on anthocyanin and amygdalin biosynthesis in sweet cherry
作者：Feng, Chen;Guo, Qingqing;Wu, Chuanbao;Wang, Jing;Zhang, Xiaoming;Yan, Guohua;Zhou, Yu;Wang, Wei;Zhang, Kaichun;Duan, Xuwei;Feng, Chen;Wu, Chuanbao;Wang, Jing;Zhang, Xiaoming;Yan, Guohua;Zhou, Yu;Wang, Wei;Zhang, Kaichun;Duan, Xuwei;Feng, Chen;Wu, Chuanbao;Wang, Jing;Zhang, Xiaoming;Yan, Guohua;Zhou, Yu;Wang, Wei;Zhang, Kaichun;Duan, Xuwei;Feng, Chen;Wu, Chuanbao;Wang, Jing;Zhang, Xiaoming;Yan, Guohua;Zhou, Yu;Wang, Wei;Zhang, Kaichun;Duan, Xuwei;Guo, Qingqing;Xue, Zheyong
关键词：Sweet cherry; UDP-glycosyltransferase; PavUGT48; Anthocyanin; Amygdalin
-
Effects of different rootstocks on fruit quality and non-volatile flavor-related compounds of sweet cherry 'summit'
作者：Wang, Naiyu;Zhang, Xiaoming;Guo, Qingqing;Yan, Guohua;Wang, Jing;Wu, Chuanbao;Zhou, Yu;Zhou, Junman;Zhang, Kaichun;Duan, Xuwei;Wang, Naiyu;Guo, Qingqing;Li, Tianzhong;Wang, Naiyu;Zhang, Xiaoming;Guo, Qingqing;Yan, Guohua;Wang, Jing;Wu, Chuanbao;Zhou, Yu;Zhou, Junman;Zhang, Kaichun;Duan, Xuwei;Wang, Naiyu;Zhang, Xiaoming;Guo, Qingqing;Yan, Guohua;Wang, Jing;Wu, Chuanbao;Zhou, Yu;Zhou, Junman;Zhang, Kaichun;Duan, Xuwei;Zhou, Junman
关键词：Prunus avium; Fertilization; Grafting; Sugar; Organic acid; Phenolic acid
-
Population sequencing of cherry accessions unravels the evolution of Cerasus species and the selection of genetic characteristics in edible cherries (vol 5, 6, 2025)
作者：Lei, Yahui;Jiu, Songtao;Xu, Yan;Lv, Zhengxin;Liu, Xunju;Wang, Lei;Wang, Li;Wang, Jiyuan;Zhang, Zhuo;Wang, Shiping;Zhang, Caixi;Lei, Yahui;Dong, Xiao;Dong, Yang;Chen, Baozheng;Dong, Yang;Bernard, Anthony;Barreneche, Teresa;Elisabeth, Dirlewanger;Cai, Yuliang;Zheng, Wei;Zhang, Xu;Li, Fangdong;Li, Hongwen;Liu, Congli;Li, Ming;Wang, Jing;Zhu, Jijun;Peng, Lei;Yu, Fei;Duan, Shengchang
关键词：
