RPT: An integrated root phenotyping toolbox for segmenting and quantifying root system architecture
文献类型： 外文期刊
作者： Shi, Jiawei 1 ; Xie, Shangyuan 1 ; Li, Weikun 1 ; Wang, Xin 1 ; Wang, Jianglin 1 ; Chen, Yunyu 1 ; Chang, Yongyue 1 ; Lou, Qiaojun 3 ; Yang, Wanneng 1 ;
作者机构： 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Hubei Hongshan Lab, Wuhan, Peoples R China
2.Huazhong Agr Univ, Natl Ctr Plant Gene Res Wuhan, Hubei Hongshan Lab, Wuhan, Peoples R China
3.Shanghai Agrobiol Gene Ctr, Shanghai, Peoples R China
4.Zhejiang Acad Agr Sci, Hangzhou, Zhejiang, Peoples R China
关键词： deep learning; high-throughput phenotyping platform; rice drought resistance; root phenotyping; root system architecture
期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：10.5； 五年影响因子：12.4 ）
ISSN： 1467-7644
年卷期： 2025 年 23 卷 6 期
页码：
收录情况： SCI
摘要： The dissection of genetic architecture for rice root system is largely dependent on phenotyping techniques, and high-throughput root phenotyping poses a great challenge. In this study, we established a cost-effective root phenotyping platform capable of analysing 1680 root samples within 2 h. To efficiently process a large number of root images, we developed the root phenotyping toolbox (RPT) with an enhanced SegFormer algorithm and used it for root segmentation and root phenotypic traits. Based on this root phenotyping platform and RPT, we screened 18 candidate (quantitative trait loci) QTL regions from 219 rice recombinant inbred lines under drought stress and validated the drought-resistant functions of gene OsIAA8 identified from these QTL regions. This study confirmed that RPT exhibited a great application potential for processing images with various sources and for mining stress-resistance genes of rice cultivars. Our developed root phenotyping platform and RPT software significantly improved high-throughput root phenotyping efficiency, allowing for large-scale root trait analysis, which will promote the genetic architecture improvement of drought-resistant cultivars and crop breeding research in the future.
- 相关文献
作者其他论文 更多>>
-
Insect residual streams supplement improves chili pepper growth: Insights into the role of rhizosphere soil microbiome and metabolome
作者：Zhou, Hanghai;Zheng, Xusong;Shen, Qi;Yao, Xiaohong;Sun, Hong;Wang, Xin;Wu, Yifei;Tang, Jiangwu;Zhu, Zhigang;Yang, Chenghu;Li, Hua;Liu, Yuxue;Jiang, Lijia;Zhang, Chunfang
关键词：Insect farming; Insect residual stream; Chili pepper; Plant growth; Rhizosphere microbial community; Metabolic profile
-
Integrated Analysis of the Transcriptome and Metabolome Reveals the Network Regulating Fruit Taste in Sponge Gourd (Luffa cylindrica)
作者：Chai, Yaqian;Qiu, Wenjing;Gao, Luyao;Dong, Wenqi;Zhang, Peng;Zhou, Shengjun;Wang, Xin;Zhu, Yuqiang;Sun, Yuyan;Chai, Yaqian;Li, Zhikun
关键词：sponge gourd; taste; transcriptome; metabolome; mechanism
-
Enhancing Akkermansia growth via phytohormones: a strategy to modulate the gut-bone axis in postmenopausal osteoporosis therapy
作者：Zhao, Zhiqi;Zhu, Liying;Wang, Xin;Li, Xiaoqiong;Li, Jinjun;Zhao, Zhiqi;Zhu, Liying;Wang, Xin;Li, Xiaoqiong;Li, Jinjun;Zhao, Zhiqi;Sun, Haibiao;Han, Xiaoqiang;Deng, Yixuan;Li, Li
关键词：Osteoporosis; Phytohormones; Soybean isoflavone; Naringin; Denosumab; Gut microbiome
-
Raffinose-metabolizing bacteria impair radiation-associated hematopoietic recovery via the bile acid/FXR/NF-κB signaling pathway
作者：Jiao, Yang;Ren, Jiawei;Yuan, Nan;Shen, Jiaqi;Guo, Hongjuan;Cao, Jianping;Wu, Depei;Jiao, Yang;Ren, Jiawei;Yuan, Nan;Guo, Hongjuan;Cao, Jianping;Jiao, Yang;Wu, Depei;Qi, Xiaofei;Xie, Shichang;Zhou, Zhemin;Shen, Jiaqi;Wu, Depei;Qi, Xiaofei;Shen, Jiaqi;Wu, Depei;Qi, Xiaofei;Shen, Jiaqi;Wu, Depei;Yin, Huafang;Wang, Jian;Wang, Xin;Wang, Xin;Zhou, Zhemin;Zhou, Zhemin
关键词：Radiation-induced hematopoietic injury; gut microbiome;
Bacteroides acidifaciens ; raffinose-metabolizing bacteria; Intestinal-Hematopoietic Axis; UDCA; FXR -
Soil heterogeneity influences the biotoxicity of photoaged tire wear particles in Eisenia fetida: A comparative assessment
作者：Zhou, Hanghai;Wang, Xin;Sun, Hong;Li, Hua;Yao, Xiaohong;Wang, Yuan;Jin, Junwei;Tang, Jiangwu;Wu, Zhou;Li, Yongfu;Zhou, Jianjiang;Jiang, Lijia;Zhang, Chunfang
关键词：Tire wear particle; Photoaging; Earthworm; Soil heterogeneity; Oxidative damage
-
Regulatory Roles of Quercetin in Alleviating Fructose-Induced Hepatic Steatosis: Targeting Gut Microbiota and Inflammatory Metabolites
作者：Li, Jinjun;Li, Xiaoqiong;Li, Jinjun;Li, Xiaoqiong;Li, Jinjun;Zhao, Zhiqi;Li, Xinxin;Zhu, Liying;Wang, Xin;Li, Xiaoqiong;Li, Jinjun;Deng, Yixuan;Li, Li
关键词：fructose; gut microbiota; hepatic steatosis; quercetin
-
6PPD-quinone exposure induces oxidative damage and physiological disruption in Eisenia fetida: An integrated analysis of phenotypes, multi-omics, and intestinal microbiota
作者：Zhou, Hanghai;Wang, Xin;Sun, Hong;Li, Hua;Wang, Yuan;Yao, Xiaohong;Tang, Jiangwu;Wu, Zhou;Wu, Zhou;Jiang, Lijia;Zhang, Chunfang;Yan, Zhongyong
关键词：Earthworm; 6PPD-quinone; Oxidative stress; Multi-omics; Intestinal microbial community
