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SlERF.J2 reduces chlorophyll accumulation and inhibits chloroplast biogenesis and development in tomato leaves

文献类型： 外文期刊

作者： Chen, Yanan 1 ; Cai, Xi 1 ; Tang, Boyan 1 ; Xie, Qiaoli 1 ; Chen, Guoping 1 ; Chen, Xuqing 2 ; Hu, Zongli 1 ;

作者机构： 1.Chongqing Univ, Bioengn Coll, Lab Mol Biol tomato, Chongqing, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Grassland Flowers & Ecol, Beijing, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Inst Grassland Flowers & Ecol, Post Box 2449,11 Shuguanghuayuan Middle Rd, Beijing 100097, Peoples R China

4.Chongqing Univ, Bioengn Coll, Room 521,Campus B,174 Shapingba Main St, Chongqing 400030, Peoples R China

关键词： Chloroplast development; Chlorophyll; SlERF; J2; (COP1); Nr mutants; Tomato; CONSTITUTIVE PHOTOMORPHOGENIC 1

期刊名称：PLANT SCIENCE （ 影响因子：5.2； 五年影响因子：5.7 ）

ISSN： 0168-9452

年卷期： 2023 年 328 卷

页码：

收录情况： SCI

摘要： Chlorophyll metabolism and chloroplast biogenesis in tomato (Solanum lycopersicum) leaves contribute to photosynthesis; however, their molecular mechanisms are poorly understood. In this study, we found that overexpression of SlERF.J2 (ethylene transcription factor) resulted in a decrease in leaf chlorophyll content and reduced accumulation of starch and soluble sugar. The slerf.j2 knockout mutant showed no apparent change. Further observation of tissue sections and transmission electron microscopy (TEM) showed that SlERF.J2 was involved in chlorophyll accumulation and chloroplast formation. RNA-seq of mature SlERF.J2-OE leaves showed that many genes involved in chlorophyll biosynthesis and chloroplast formation were significantly downregulated compared with those in WT leaves. Genome global scanning of the ERF TF binding site combined with RNA-seq differential gene expression and qRT-PCR detection analysis showed that COP1 was a potential target gene of SlERF.J2. Tobacco transient expression technology, a dual-luciferase reporter system and Y1H technology were employed to verify that SlERF.J2 could bind to the COP1 promoter. Notably, overexpression of SlERF.J2 in Nr mutants resulted in impaired chloroplast biogenesis and development. Taken together, our findings demonstrated that SlERF.J2 plays an essential role in chlorophyll accumulation and chloroplast formation, laying a foundation for enhancing plant photosynthesis.

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