文献类型： 外文期刊

作者： Qian, Linlin ¹ ; Jin, Hangxia ¹ ; Yang, Qinghua ¹ ; Zhu, Longming ¹ ; Yu, Xiaomin ¹ ; Fu, Xujun ¹ ; Zhao, Man ³ ; Yuan, Fengjie ¹ ;

作者机构： 1.Zhejiang Acad Agr Sci, Hangzhou Sub Ctr Natl Soybean Improvement, Inst Crop & Nucl Technol Utilizat, Hangzhou 310021, Peoples R China

2.Minist Agr & Rural Affairs China, Key Lab Informat Traceabil Agr Prod, Hangzhou 310021, Peoples R China

3.Zhejiang Univ Technol, Coll Biotechnol & Bioengn, Natl & Local Joint Engn Res Ctr Biomfg Chiral Che, Hangzhou 310014, Peoples R China

4.Zhejiang Acad Agr Sci, Zhejiang Key Lab Digital Dry Land Crops, Hangzhou 310021, Peoples R China

关键词： aromatic vegetable soybean; gene sequence comparison; HRM molecular marker; soybean breeding; CRISPR; Cas9 gene editing

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 8 期

页码：

收录情况： SCI

摘要： The vegetable soybean (Glycine max L. Merr.) plant is commonly consumed in Southeast Asian countries because of its nutritional value and desirable taste. A "pandan-like" aroma is an important value-added quality trait that is rarely found in commercial vegetable soybean varieties. In this study, three novel aromatic soybean cultivars with a fragrant volatile compound were isolated. We confirmed that the aroma of these cultivars is due to the potent volatile compound 2-acetyl-1-pyrroline (2AP) that was previously identified in soybean. A sequence comparison of GmBADH1/2 (encoding an aminoaldehyde dehydrogenase) between aromatic and non-aromatic soybean varieties revealed a mutation with 10 SNPs and an 11-nucleotide deletion in exon 1 of GmBADH2 in Quxian No. 1 and Xiangdou. Additionally, a 2-bp deletion was detected in exon 10 of GmBADH2 in ZK1754. The mutations resulted in a frame shift and the introduction of premature stop codons. Moreover, genetic analyses indicated that the aromatic trait in these three varieties was inherited according to a single recessive gene model. These results suggested that a mutated GmBADH2 may be responsible for the aroma of these three aromatic soybean cultivars. The expression and function of GmBADH2 in aromatic soybean seeds were confirmed by qRT-PCR and CRISPR/Cas9. A functional marker developed on the basis of the mutated GmBADH2 sequence in Quxian No. 1 and Xiangdou was validated in an F-2 population. A perfect association between the marker genotypes and aroma phenotypes implied that GmBADH2 is a major aroma-conferring gene. The results of this study are potentially useful for an in-depth analysis of the molecular basis of 2-AP formation in soybean and the marker-assisted breeding of aromatic vegetable soybean cultivars.