Kinetics and thermodynamics of glucoamylase inhibition by lactate during fermentable sugar production from food waste
文献类型： 外文期刊
作者： Wang, Xiao Qiang 2 ; Wang, Qun Hui 1 ; Liu, Ying Ying 1 ; Ma, Hong Zhi 1 ; Wang, Xu Ming 3 ;
作者机构： 1.Beijing Univ Sci & Technol, Dept Environm Engn, Beijing 100083, Peoples R China
2.N China Elect Power Univ, Natl Engn Lab Biomass Power Generat Equipment, Sch Renewable Energy, Beijing 102206, Peoples R China
3.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100089, Peoples R China
关键词： glucoamylase;Aspergillus niger UV-60;lactate;food waste;inhibition;denaturation
期刊名称：JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY （ 影响因子：3.174； 五年影响因子：3.137 ）
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收录情况： SCI
摘要： BACKGROUND: Glucoamylase hydrolysis is a key step in the bioconversion of food waste with complicated composition. This work investigated the effect of lactate on glucoamylase from Aspergillus niger UV-60, and inhibition mechanisms of glucoamylase by lactate during food waste hydrolysis. RESULTS: For 125 min hydrolysis of food waste (10%, dry basis), reducing sugars produced in the absence of lactate were 15%, 26% and 56% more than those produced in the presence of 24 g L1 lactate at 60, 50 and 40 C, respectively. Kinetic study showed that the type of glucoamylase inhibition by lactate was competitive, and Km (Michaelis-Menten constent), Vmax (maximum initial velocity), KI (inhibition constant) were 103.2 g L1, 5.0gL1 min1, 100.6 g L1, respectively, for food waste hydrolysis at 60 C and pH 4.6. Lactate also accelerated glucoamylase denaturation significantly. Activation energy of denaturation without inhibitor was 61% greater than that of denaturation with inhibitor (24 g L1 lactate). Half-lives (t1/2) without inhibitor were 7.6, 2.7, 2.6, 1.7 and 1.2 times longer than those with inhibitor at temperature 40, 45, 50, 55 and 60 C, respectively.
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