Establishment of virus-induced gene silencing (VIGS) system in Luffa acutangula using Phytoene desaturase (PDS) and tendril synthesis related gene (TEN)
文献类型： 外文期刊
作者： Qi, Xiaoyu 1 ; Mo, Qiaoping 1 ; Li, Jing 1 ; Zi, Zhibo 1 ; Xu, Mengyun 1 ; Yue, Suju 4 ; Zhao, Hongbo 1 ; Zhu, Haisheng 5 ; Wang, Guoping 1 ;
作者机构： 1.South China Agr Univ, Coll Hort, Guangzhou 510642, Guangdong, Peoples R China
2.South China Agr Univ, Coll Hort, Key Lab Biol & Germplasm Enhancement Hort Crops So, Minist Agr, Guangzhou 510642, Guangdong, Peoples R China
3.Tibet Agr & Anim Husb Univ, Coll Plant Sci, Nyingchi 860000, Tibet, Peoples R China
4.Zhongkai Univ Agr & Engn, Coll Life Sci, Guangzhou 510225, Guangdong, Peoples R China
5.Fujian Acad Agr Sci, Crop Res Inst, Fujian Key Lab Vegetable Genet & Breeding, Fuzhou 350013, Fujian, Peoples R China
关键词： Luffa; VIGS; Cucumber green mottle mosaic virus (CGMMV); PDS; TEN
期刊名称：PLANT METHODS （ 影响因子：5.1； 五年影响因子：6.1 ）
ISSN：
年卷期： 2023 年 19 卷 1 期
页码：
收录情况： SCI
摘要： Background Virus-induced gene silencing (VIGS) is a reverse genetics technology that can efficiently and rapidly identify plant gene functions. Although a variety of VIGS vectors have been successfully used in plants, only a few reports on VIGS technology in Luffa exist.Results In the present study, a new cucumber green mottle mosaic virus (CGMMV)-based VIGS vector, pV190, was applied to establish the CGMMV-VIGS to investigate the feasibility of the silencing system for Luffa. Phytoene desaturase (PDS) gene was initially selected as a VIGS marker gene to construct a recombinant vector. Plants infected with Agrobacterium harboring pV190-PDS successfully induced effective silencing in Luffa, and an effective gene silencing phenotype with obvious photobleaching was observed. To further validate the efficiency, we selected TEN for gene-silencing, which encodes a CYC/TB1-like transcription factor and is involved in tendril development. Luffa plants inoculated with the pV190-TEN exhibited shorter tendril length and nodal positions where tendrils appear are higher compared to those of non-inoculated plants. RT-qPCR showed that the expression levels of PDS and TEN were significantly reduced in the CGMMV-VIGS plants. Moreover, we evaluated the CGMMV-VIGS efficiency in three cucurbits, including cucumber, ridge gourd, and bottle gourd.Conclusion We successfully established a CGMMV-based VIGS system on ridge gourd and used marker genes to identify the feasibility of the silencing system in Luffa leaves and stems.
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