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Ethylene response factors regulate expression of HbSUT3, the sucrose influx carrier in laticifers of Hevea brasiliensis

文献类型： 外文期刊

作者： Zhang, Yi 1 ; Xin, Lusheng 1 ; Pirrello, Julien 3 ; Fang, Yongjun 5 ; Yang, Jianghua 5 ; Qi, Jiyan 1 ; Montoro, Pascal 3 ;

作者机构： 1.Hainan Univ, Coll Trop Crops, Nat Rubber Cooperat Innovat Ctr Hainan Prov, 58 Renmin Ave, Haikou 570228, Hainan, Peoples R China

2.Hainan Univ, Coll Trop Crops, Minist Educ PRC, 58 Renmin Ave, Haikou 570228, Hainan, Peoples R China

3.UMR AGAP, CIRAD, 389 Ave Agropolis TA A-108-03, F-34398 Montpellier, France

4.Univ Montpellier, Montpellier SupAgro, INRA, CIRAD,AGAP, 389 Ave Agropolis TA A-108-03, F-34398 Montpellier, France

5.Chinese Acad Trop Agr Sci, Rubber Res Inst, 4 West Xueyuan Rd, Haikou 570100, Hainan, Peoples R China

关键词： ethylene signaling; gene expression; rubber biosynthesis; sucrose transporter; transcription factor

期刊名称：TREE PHYSIOLOGY （ 影响因子：4.196； 五年影响因子：4.727 ）

ISSN： 0829-318X

年卷期： 2021 年 41 卷 7 期

页码：

收录情况： SCI

摘要： Natural rubber is an important industrial raw material and is commercially produced by rubber trees (Hevea brasiliensis). The sucrose transporter HbSUT3 plays an essential role in rubber production. Its expression in latex (cytoplasm of rubber-producing laticifers) is induced by bark treatment with Ethrel, an ethylene releaser, and the inducing effect correlates well with Ethrel-stimulated rubber yield increase. However, the mechanisms of ethylene induction on HbSUT3 expression are not known. Here, five Ethylene Response Factor (ERF) genes were identified from the cDNA library of Hevea latex by yeast one-hybrid screening with the promoter of HbSUT3 gene as bait. As revealed in a tobacco (Nicotiana tabacum) protoplast transient expression system, these HbERFs were mainly localized in the nucleus and four of them exhibited apparent transactivation activity. Of the five HbERF genes, HbERF-IXc4 was the most frequently screened in yeast one-hybrid, accounting for 65% of the ERF clones obtained. Moreover, among the five HbERFs, HbERF-IXc4 showed the strongest transactivation capacity when expressed in tobacco protoplast, the highest transcript abundance in latex and a close expressional correlation with its target gene, HbSUT3, in response to the Ethrel treatment. Taken together, our results indicate that ERFs, especially HbERF-IXc4, are critically involved in the activation of HbSUT3 expression in latex after Ethrel treatment on Hevea bark, and thus the stimulated latex yield.

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