cDNA Cloning, Prokaryotic Expression, Polyclonal Antibody Preparation of the Auxin-Binding Protein 1 Gene from Grape Berry
文献类型： 外文期刊
作者： Wan, Si-Bao 1 ; Wang, Wei 1 ; Luo, Mei 1 ; Huang, Wei-Dong 1 ; Yin, Jing-Yuan 2 ; Zhan, Ji-Cheng 1 ;
作者机构： 1.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China
2.Shanghai Univ, Coll Life Sci, Shanghai 200444, Peoples R China
3.Beijing Acad Agr & Forestry Sci, Inst Forestry & Pomol, Beijing 100093, Peoples R China
关键词： amino acid sequence;prokaryotic expression;carboxyl-terminal sorting signal
期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Auxin-binding protein 1 (ABP1) plays an important role in the growth and development of plants. In this study, a novel grape ABP1 cDNA was isolated by reverse transcription polymerase chain reaction, using in silico cloning strategy based on grape dbEST. The obtained grape ABP1 cDNA is 756 bp containing a 567 bp open reading frame, which encodes a 188 amino acid protein. The deduced amino acid sequence possessed all of the three typical domains of plant ABP1s, an amino-terminal signal peptide and a carboxyl-terminal sorting signal, KDEL. A full-length ABP1 cDNA was introduced into an expressed plasmid pET-30a(+) vector at the EcoR I and Xho I restriction sites. The pET-ABP1 was found to be highly expressed in Escherichia coli BL21(DE3) pLysS cells with isopropyl-beta-d-thiogalactoside induction. A fusion protein was purified and used as the antigen to immunize a New Zealand rabbit. The resulting antiserum was then further purified by HiTrap rProtein A FF Affinity Purification Kit to obtain the immunoglobulin G fraction. Western blot analysis indicated that ABP1 was regulated in fruits depending on the developmental stage. Our work represented a first step towards a better understanding of function analysis of grape ABP1.
- 相关文献
作者其他论文 更多>>
-
Effects of maternal Escherichia coli lipopolysaccharide exposure on offspring: insights from lncRNA analysis in laying hens
作者：Yan, Zhixun;Liu, Huagui;Chu, Qin;Liu, Lei;Liu, Lei;Yu, Ying;Wang, Wei;Adetula, Adeyinka Abiola
关键词：LPS maternal stimulation; Chicken; Offspring; Egg-laying rate; lncRNA
-
Fluorinated covalent triazine frameworks for highly efficient removal of per- and polyfluoroalkyl substances in water: Synthesis, performance and mechanism
作者：Gong, Wenwen;Gong, Minjuan;Xiao, Guotao;Zhang, Ruitong;Zhu, Xingyi;Li, Deyan;Zhou, Shuangxi;Wang, Wei;Li, Deyan
关键词：Covalent triazine frameworks; Synthesis; PFAS; Performance; Sorption mechanism
-
Improving the stability of black soil microbial communities through long-term application of biochar to optimize the characteristics of DOM components
作者：Hu, Yu;Liu, Kangmeng;Yang, Zhenguo;Xu, Kuifeng;Yan, Lilong;Li, Shuo;Jin, Liang;Wei, Dan;Li, Yan;Wang, Wei;Shi, Chuanqi;Wang, Yuxian
关键词：Biochar; Black soil; DOM fluorescent components; Sampling period; Microplastics; Network analysis
-
Prioritized SNP Selection from Whole-Genome Sequencing Improves Genomic Prediction Accuracy in Sturgeons Using Linear and Machine Learning Models
作者：Song, Hailiang;Wang, Wei;Dong, Tian;Yan, Xiaoyu;Geng, Chenfan;Bai, Song;Hu, Hongxia;Song, Hailiang;Wang, Wei;Dong, Tian;Yan, Xiaoyu;Geng, Chenfan;Bai, Song;Hu, Hongxia;Song, Hailiang;Wang, Wei;Dong, Tian;Yan, Xiaoyu;Geng, Chenfan;Bai, Song;Hu, Hongxia
关键词：sturgeon; genomic prediction; GWAS; machine learning models; SNP density; aquaculture industry; breeding; caviar traits
-
Fungal diversity notes 1818-1918: taxonomic and phylogenetic contributions on genera and species of fungi
作者：Manawasinghe, Ishara S.;Hyde, Kevin D.;Xia, Guiyang;Senanayake, Indunil C.;Liu, Jia-Wei;Lin, Jie-Ying;Luo, Mei;Doilom, Mingkwan;Kularathnage, Nuwan D.;Dong, Wei;Xiong, Yinru;Shu, Yongxin;Zhang, Yun Xia;Song, Jia Ge;Xu, Biao;Manawasinghe, Ishara S.;Hyde, Kevin D.;Liu, Jia-Wei;Doilom, Mingkwan;Kularathnage, Nuwan D.;Song, Jia Ge;Xu, Biao;Manawasinghe, Ishara S.;Hyde, Kevin D.;Liu, Jia-Wei;Lin, Jie-Ying;Doilom, Mingkwan;Kularathnage, Nuwan D.;Zhou, Yueyan;Madushani, Manawaduge Ayesha;Manawasinghe, Ishara S.;Hyde, Kevin D.;Armand, Alireza;Li, Cuijinyi;Xia, Guiyang;Ma, Jian;Phonemany, Monthien;Thongklang, Naritsada;Kularathnage, Nuwan D.;Xu, Rong-Ju;Xu, Rui-Fang;Liu, Xiang-Fu;Peng, Xing-Can;Yang, Yanyan;Gao, Ying;Xiong, Yinru;Shu, Yongxin;Madushani, Manawaduge Ayesha;Wanasinghe, Dhanushka N.;Wanasinghe, Dhanushka N.;Karunarathna, Samantha C.;Lu, Li;Du, Tian-Ye;Lu, Wen-Hua;Karunarathna, Samantha C.;Maharachchikumbura, Sajeewa S. N.;Maharachchikumbura, Sajeewa S. N.;Samarakoon, Milan C.;Samarakoon, Milan C.;Coleine, Claudia;Coleine, Claudia;Liimatainen, Kare;Niskanen, Tuula;Liimatainen, Kare;Niskanen, Tuula;He, Shu-Cheng;Gajanayake, Achala Jeevani;Armand, Alireza;Xia, Guiyang;Shen, Hong-Wei;Phonemany, Monthien;Thongklang, Naritsada;Kularathnage, Nuwan D.;Jayawardena, Ruvishika S.;Du, Tian-Ye;Liu, Xiang-Fu;Peng, Xing-Can;Yang, Yanyan;Gao, Ying;Xiong, Yinru;Shu, Yongxin;Zhou, Yueyan;Boonmie, Saranyapath;Tsurykau, Andrei;Tsurykau, Andrei;de Medeiros, Erika Valente;Li, Cuijinyi;Li, Cuijinyi;Denchev, Cvetomir M.;Denchev, Teodor T.;Denchev, Cvetomir M.;Kemler, Martin;Denchev, Teodor T.;Wei, De-Ping;Wei, De-Ping;Kemler, Martin;Langer, Ewald;Zhang, Fa;Shen, Hong-Wei;Luo, Zong-Long;de Souza, Francisco A.;Magurno, Franco;Barreto, Gabriel G.;Gusmao, Luis F. P.;Mane, Gajanan;da Silva, Gladstone Alves;de Oliveira, Thays Gabrielle Lins;Ma, Jian;Lu, Yong-Zhong;dos Santos, Lidiane A.;Caceres, Marcela E. S.;Ding, Mengmeng;Du, Tingting;Wu, Tong;Camara, Marcos Paz Saraiva;de Barros-Barreto, Maria B. B.;Calabon, Mark S.;Kukwa, Martin;Ghobad-Nejhad, Masoomeh;Zhao, Qi;Xu, Rong-Ju;Alfenas, Rafael Ferreira;de Oliveira, Rafael J. V.;Singh, Raghvendra;Rajwar, Soumyadeep;da Silva, Rejane Maria Ferreira;Avchar, Rameshwar;Sharma, Rohit;Morey, Rashmi;da Silveira, Rosa Mara B.;Jayawardena, Ruvishika S.;Nanu, Salna;dos Vieira, Willie Anderson;Varghese, Sherin;Moreira, Silvino Intra;He, Shu-Cheng;He, Shu-Cheng;Kumar, T. K. Arun;Wen, Ting-Chi;Peng, Xing-Can;Wen, Ting-Chi;Peng, Xing-Can;Doyle, Vinson P.;Baulin, Vladimir;Zhang, Xian;Lu, Wen-Hua;von Brackel, Wolfgang;Yang, Yanyan;Yang, Yanyan;Shen, Yuan-Min
关键词：71 new species; 71 new species; 21 new records; 21 new records; 4 new genera; 4 new genera; Ascomycota; Ascomycota; Basidiomycota; Basidiomycota; Dothideomycetes; Dothideomycetes; Fossil fungi; Fossil fungi; Lecanoromycetes; Lecanoromycetes; Leotiomycetes; Leotiomycetes; Glomeromycota; Glomeromycota; Sordariomycetes; Sordariomycetes
-
Identification and characteristic analysis of PavUGT48 as a novel UDP-glycosyltransferase with dual functions on anthocyanin and amygdalin biosynthesis in sweet cherry
作者：Feng, Chen;Guo, Qingqing;Wu, Chuanbao;Wang, Jing;Zhang, Xiaoming;Yan, Guohua;Zhou, Yu;Wang, Wei;Zhang, Kaichun;Duan, Xuwei;Feng, Chen;Wu, Chuanbao;Wang, Jing;Zhang, Xiaoming;Yan, Guohua;Zhou, Yu;Wang, Wei;Zhang, Kaichun;Duan, Xuwei;Feng, Chen;Wu, Chuanbao;Wang, Jing;Zhang, Xiaoming;Yan, Guohua;Zhou, Yu;Wang, Wei;Zhang, Kaichun;Duan, Xuwei;Feng, Chen;Wu, Chuanbao;Wang, Jing;Zhang, Xiaoming;Yan, Guohua;Zhou, Yu;Wang, Wei;Zhang, Kaichun;Duan, Xuwei;Guo, Qingqing;Xue, Zheyong
关键词：Sweet cherry; UDP-glycosyltransferase; PavUGT48; Anthocyanin; Amygdalin
-
Characterization of volatile profiles in cherry fruits: Integration of E-nose and HS-SPME-GC-MS
作者：Feng, Chen;Zhang, Xiaoming;Wang, Jing;Wu, Chuanbao;Wang, Wei;Yan, Guohua;Zhou, Yu;Zhang, Kaichun;Duan, Xuwei;Feng, Chen;Zhang, Xiaoming;Wang, Jing;Wu, Chuanbao;Wang, Wei;Yan, Guohua;Zhou, Yu;Zhang, Kaichun;Duan, Xuwei;Feng, Chen;Zhang, Xiaoming;Wang, Jing;Wu, Chuanbao;Wang, Wei;Yan, Guohua;Zhou, Yu;Zhang, Kaichun;Duan, Xuwei;Feng, Chen;Zhang, Xiaoming;Wang, Jing;Wu, Chuanbao;Wang, Wei;Yan, Guohua;Zhou, Yu;Zhang, Kaichun;Duan, Xuwei
关键词：Cherry; E -nose; HS-SPME-GC-MS; Volatile compounds; Relative odor activity value
