您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A newly identified glycosyltransferase AsRCOM provides resistance to purple curl leaf disease in agave

文献类型： 外文期刊

作者： Lu, Zhiwei 1 ; Hou, Xiaowan 4 ; Ke, Zhi 1 ; Zhang, Yanmei 1 ; Yang, ZiPing 1 ; Zhou, Wenzhao 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, South Subtrop Crops Inst, Zhanjiang City Key Lab Trop Crops Genet Improvemen, Zhanjiang 524091, Guangdong, Peoples R China

2.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China

3.Chinese Acad Agr Sci CAAS, Inst Crop Sci, Key Lab Biol & Genet Improvement Triticeae Crops, Natl Key Facil Crop Gene Resources & Genet Improve, Beijing 100081, Peoples R China

4.Chinese Acad Trop Agr Sci, South Subtrop Crops Res Inst, Key Lab Postharvest Physiol & Technol Trop Hort Pr, Zhanjiang 524091, Guangdong, Peoples R China

关键词： Agave; Resistant mechanism; AsRCOM gene; Hypersensitive response

期刊名称：BMC GENOMICS （ 影响因子：4.4； 五年影响因子：4.7 ）

ISSN： 1471-2164

年卷期： 2023 年 24 卷 1 期

页码：

收录情况： SCI

摘要： Background Purple curl leaf disease brings a significant threat to the development of agave industry, the underlying mechanism of disease-resistant Agave sisalana. hybrid 11648 (A. H11648R) is still unknown.Results To excavate the crucial disease-resistant genes against purple curl leaf disease, we performed an RNA-seq analysis for A.H11648R and A.H11648 during different stages of purple curl leaf disease. The DEGs (differentially expressed genes) were mainly enriched in linolenic acid metabolism, starch and sucrose mechanism, phenylpropanoid biosynthesis, hypersensitive response (HR) and systemic acquired resistance. Further analysis suggested that eight candidate genes (4'OMT2, ACLY, NCS1, GTE10, SMO2, FLS2, SQE1 and RCOM) identified by WGCNA (weighted gene co-expression network analysis) may mediate the resistance to agave purple curl disease by participating the biosynthesis of benzylisoquinoline alkaloids, steroid, sterols and flavonoids, and the regulation of plant innate immunity and systemic acquired resistance. After qPCR verification, we found that AsRCOM, coding a glycosyltransferase and relevant to the regulation of plant innate immunity and systemic acquired resistance, may be the most critical disease-resistant gene. Finally, the overexpression of AsRCOM gene in agave could significantly enhance the resistance to purple curl disease with abundant reactive oxygen species (ROS) accumulations.Conclusions Integrative RNA-seq analysis found that HR may be an important pathway affecting the resistance to purple curl leaf disease in agave, and identified glycosyltransferase AsRCOM as the crucial gene that could significantly enhance the resistance to purple curl leaf disease in agave, with obvious ROS accumulations.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台