文献类型： 外文期刊

作者： Wang, Hongzhen ¹ ; Chen, Xinying ² ; Yan, Xiaoyun ¹ ; Xu, Zhixia ¹ ; Shao, Qingsong ¹ ; Wu, Xueqian ¹ ; Tou, Lingjuan ³ ; Fang, Li ³ ; Wei, Mingming ⁴ ; Wang, Huasen ² ;

作者机构： 1.Zhejiang Agr & Forestry Univ, State Key Lab Subtrop Silviculture, Zhejiang Prov Key Lab Resources Protect & Innovat, Dept Chinese Herbal Med, Hangzhou 311300, Peoples R China

2.Zhejiang Agr & Forestry Univ, Key Lab Qual & Safety Control Subtrop Fruits & Ve, Collaborat Innovat Ctr Efficient & Green Prod Agr, Minist Agr & Rural Affairs,Coll Hort Sci, Hangzhou 311300, Peoples R China

3.Jinhua Acad Agr Sci, Jinhua 321017, Peoples R China

4.Chinese Acad Trop Agr Sci, State Key Lab Breeding Base Cultivat & Physiol Tr, Key Lab Biol & Genet Resource Utilizat Rubber Tre, Minist Agr,Rubber Res Inst, Danzhou 571700, Peoples R China

关键词： Anoectochilus roxburghii (Wall.) Lindl; IPR-PLB; propagation; kinsenoside; flavonoids

期刊名称：PLANTS-BASEL （ 影响因子：4.658； 五年影响因子：4.827 ）

ISSN：

年卷期： 2022 年 11 卷 19 期

页码：

收录情况： SCI

摘要： Anoectochilus roxburghii (Wall.) Lindl has been used in Chinese herbal medicine for treating various ailments. However, its wild resources are endangered, and artificial cultivation of the plant is limited by the low regeneration rate of conventional propagation methods. The lack of A. roxburghii resources is detrimental to the commercial production of the plant and kinsenoside, which is unique to Anoectochilus species. To develop highly efficient methods for A. roxburghii micropropagation and find alternative resources for kinsenoside production, we created an induction, proliferation, and regeneration of PLBs (IPR-PLB) protocol for A. roxburghii. We also analyzed the kinsenoside and flavonoid contents during the induction and proliferation of PLBs. The best media of IPR-PLB for PLB induction and proliferation (secondary PLB induction and proliferation), shoot formation, and rooting medium were Murashige and Skoog (MS) + 3 mg/L 6-benzylaminopurine (6-BA) + 0.5 mg/L naphthaleneacetic acid (NAA) + 0.8 mg/L zeatin (ZT) + 0.2 mg /L 2,4-dichlorophenoxyacetic acid (2, 4-D), MS + 3 mg/L 6-BA + 0.5 mg/L NAA, and MS + 0.5 mg /L NAA, respectively. On these optimized media, the PLB induction rate was 89 +/- 2.08%, secondary PLB induction rate was 120 +/- 5%, secondary PLB proliferation rate was 400 +/- 10% and 350 +/- 10 % in terms of the quantity and biomass at approximately 1 month, shoot induction rate was 10.5 shoots/PLB mass, and root induction rate was 98%. All plantlets survived after acclimation. Darkness or weak light were essential for PLB proliferation, and light was crucial for PLB differentiation on these optimized media. The kinsenoside contents of PLBs and secondary PLBs were 10.38 +/- 0.08 and 12.30 +/- 0.08 mg/g fresh weight (FW), respectively. Moreover, the peak kinsenoside content during the proliferation of secondary PLBs was 34.27 +/- 0.79 mg/g FW, which was slightly lower than that of the whole plant (38.68 +/- 3.12 mg/g FW). Two flavonoids exhibited tissue- or temporal-specific accumulation patterns, and astragalin accumulated exclusively during the first 2 weeks of cultivation. The IPR-PLB protocol for A. roxburghii may facilitate the efficient micropropagation of A. roxburghii plants. Furthermore, the PLBs are a good alternative resource for kinsenoside production.