Identification and tissue distribution of odorant binding protein genes in Harmonia axyridis (Coleoptera: Coccinellidae)
文献类型： 外文期刊
作者： Qu Cheng 1 ; Wang Ran 2 ; Che Wu-nan 3 ; Li Feng-qi 2 ; Zhao Hai-peng 1 ; Wei Yi-yun 2 ; Luo Chen 2 ; Xue Ming 1 ;
作者机构： 1.Shandong Agr Univ, Coll Plant Protect, Tai An 271018, Shandong, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China
3.Shenyang Agr Univ, Dept Pesticide Sci, Shenyang 110866, Peoples R China
关键词： odorant-binding proteins; Harmonia axyridis; expression profile; phylogenetic tree
期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）
ISSN： 2095-3119
年卷期： 2021 年 20 卷 8 期
页码：
收录情况： SCI
摘要： The olfactory system of insects is crucial in modulating behaviors such as host seeking, mating, and oviposition. Odorant binding proteins (OBPs) are involved in semiochemical recognition. OBPs recognize and bind odorants and transport them to odorant receptors located in olfactory neurons. Harmonia axyridis (Coleoptera: Coccinellidae) is a widely used predacious biological control agent for many agricultural and forestry pests. This study identified 19 OBPs in H. axyridis based on the antennal and whole-body transcriptomes of adults and obtained all the full-length open reading frames, including 11 'Classic' OBPs, 7 'Minus-C' OBPs and 1 'Plus-C' OBP. They encoded 125 to 241 amino acid proteins with molecular weights ranging from 13.75 to 27.75 kDa and isoelectric points ranging from 4.15 to 8.80. Phylogenetic analyses were used to study the relationships between H. axyridis OBPs and OBPs from other species of Coleoptera. Quantitative real-time PCR (qPCR) analysis showed that HaxyOBP2, 3, 5, 8, 10, 12, 13, 14, and 15 were highly expressed in antennae of both adult females and males. Moreover, HaxyOBP2, 3, 5, 12, and 15 were more abundantly expressed in antennae than other body parts, while HaxyOBP13 and HaxyOBP14 were expressed predominantly, and at similar levels, in the head and antennae. The other OBP genes were highly expressed in non-olfactory tissues including the thorax, abdomen, legs, and wings. These results provide valuable information for further study of H. axyridis olfaction, which may ultimately enhance its use as a biocontrol agent.
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