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An integrated distance-based microfluidic aptasensor for visual quantitative detection of Salmonella with sample-in-answer-out capability

文献类型： 外文期刊

作者： Man, Yan 1 ; Ban, Meijing 1 ; Jin, Xinxin 1 ; Li, An 1 ; Tao, Jing 1 ; Pan, Ligang 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Qual Stand & Testing Technol, Beijing, Peoples R China

2.Minist Agr, Risk Assessment Lab Agroprod Beijing, Beijing, Peoples R China

关键词： Microfluidic chip; Distance; Colorimetric; Salmonella; Aptamer

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：8.4； 五年影响因子：7.2 ）

ISSN：

年卷期： 2023 年 381 卷

页码：

收录情况： SCI

摘要： A portable distance-based microfluidic aptasensor with sample-in-answer-out capability was designed for equipment-free, in-filed, visual quantitative detection of Salmonella by microcolumns-based vortex on-line micromixing and incubation, nitrocellulose membranes fixed with single-strand binding proteins (SSBP-NCMs)-based enrichment and separation, and distance-readout based visual quantitative detection. Specifically, aptamers modified with biotin (bio-aptamers) were used as detection probes and reacted with Salmonella typhimurium. The replaceable SSBP-NCMs with high adsorbability captured free bio-aptamers, making the microfluidic chips reusable. The formed bio-aptamer-SSBP-NCMs combined with horseradish peroxidase con-jugated streptavidin (HRP-SA) which catalyzes hydrogen peroxide (H2O2) to produce oxygen (O2). O2 pushed the red gold nanoparticles (GNPs) to move a certain distance along serpentine channel. Hence, the readable distance signals were proportional to the concentrations of Salmonella in samples. The developed distance-based micro -fluidic aptasensor can detect Salmonella typhimurium as low as 3.7 x 101 cfu mL-1 and exhibited a good spec-ificity and reliability.

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