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Neprilysin, a novel recombinant venom protein from Habrobracon hebetor, influenced host immunity in vitro

文献类型： 外文期刊

作者： Yu, Kaili 1 ; Li, Fang 1 ; Zhong, Haiying 1 ; Zhang, Bo 2 ; Fang, Qi 2 ; Xu, Hongxing 1 ; Song, Qisheng 3 ; Ye, Gongyin 2 ; Zhang, Juefeng 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, Hangzhou 310021, Zhejiang, Peoples R China

2.Zhejiang Univ, Inst Insect Sci, State Key Lab Rice Biol & Breeding, Minist Agr & Rural Affairs,Key Lab Biol Crop Patho, Hangzhou 310058, Peoples R China

3.Univ Missouri, Coll Agr Food & Nat Resources, Div Plant Sci & Technol, Columbia, MO 65211 USA

关键词： Venom protein; Neprilysin; Habrobracon hebetor; Function; Biocontrol

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 319 卷

页码：

收录情况： SCI

摘要： Ectoparasitoid wasps inject venom into their hosts during the parasitism to paralyze the hosts and inhibit their immune response and development. The venom of the parasitoid wasp, Habrobracon hebetor, contains proteins that facilitate its parasitization of various lepidopteran pests, and paralyze the 3rd to 5th instar larvae of Spodoptera litura. We comparatively analyzed the function of venom protein neprilysin (NEP) from H. hebetor in two different hosts. NEP is a highly conserved endopeptidase present in various insect tissues. H. hebetor contains 17 NEPs, but only one venom-specific NEP was identified. The venom HhNEP is composed of 771 amino acids encoded by a 2316 base open reading frame, with a molecular mass of 87.77 kDa containing a conserved M13 peptidase activity domain. It exhibits high total mRNA expression levels in the venom glands. The recombinant HhNEP, expressed in prokaryotic system, could simultaneously inhibit the prophenoloxidase (PPO) activation in the hemolymph and the encapsulation responses in the hemocytes of S. litura and Plodia interpunctella larvae in vitro. Additionally, recombinant HhNEP inhibited the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in hemocytes from two hosts. In conclusion, the venom HhNEP could inhibit the immune response of hosts.

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