Specific Nested PCR for the Detection of 16SrI and 16SrII Group Phytoplasmas Associated with Yellow Leaf Disease of Areca Palm in Hainan, China
文献类型： 外文期刊
作者： Ge, Huiyuan 1 ; Meng, Xiuli 3 ; Lin, Zhaowei 3 ; Jan, Saad 3 ; Song, Weiwei 3 ; Qin, Weiquan 3 ; Tang, Qinghua 3 ; Zhu, Xiaoqiong 1 ;
作者机构： 1.China Agr Univ, Coll Plant Protect, Dept Plant Pathol, MARA Key Lab Pest Monitoring & Green Management, Beijing, Peoples R China
2.China Agr Univ, Coll Plant Protect, MARA Key Lab Pest Monitoring & Green Management, Beijing 100193, Peoples R China
3.Chinese Acad Trop Agr Sci, Coconut Res Inst, HPDST Hainan Innovat Ctr Academician Team, Wenchang 571339, Peoples R China
4.Sanya Inst China Agr Univ, Sanya 572000, Peoples R China
关键词：
期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）
ISSN： 2223-7747
年卷期： 2025 年 14 卷 14 期
页码：
收录情况： SCI
摘要： Yellow leaf disease (YLD), caused by the areca palm yellow leaf phytoplasma (APYL), poses a significant threat to the sustainability of the areca palm industry. Timely and accurate detection is essential for effectively diagnosing and managing this disease. This study developed a novel nested PCR system using primers specifically designed from conserved regions of the phytoplasma 16S rDNA sequence to overcome limitations such as false positives often associated with universal nested PCR primers. The resulting primer pairs HNP-1F/HNP-1R (outer) and HNP-2F/HNP-2R (inner) consistently amplified a distinct 429 bp fragment from APYL strains belonging to the 16SrI and 16SrII groups. The detection sensitivity reached 7.5 x 10-7 ng/mu L for 16SrI and 4 x 10-7 ng/mu L for 16SrII. Field validation using leaf samples from symptomatic areca palms confirmed the high specificity and reliability of the new primers in detecting APYL. Compared to conventional universal primers (P1/P7 and R16mF2/R16mR1), this newly developed nested PCR system demonstrated higher specificity, sensitivity, and speed, making it a valuable tool for the early diagnosis and management of YLD in areca palms.
- 相关文献
作者其他论文 更多>>
-
Combined Analysis of Metabolomics and Biochemical Changes Reveals the Nutritional and Functional Characteristics of Red Palm Weevil Rhynchophus ferrugineus (Coleoptera: Curculionidae) Larvae at Different Developmental Stages
作者：Chen, Mengran;Kan, Jintao;Zhang, Yufeng;Zhao, Jinhao;Lv, Chaojun;Zhong, Baozhu;Li, Chaoxu;Qin, Weiquan
关键词：red palm weevil larvae; untargeted metabolomics; nutrients; antioxidant activity
-
A Putative Ormycovirus That Possibly Contributes to the Yellow Leaf Disease of Areca Palm
作者：Niu, Xiaoqing;Qin, Weiquan;Xu, Zhongtian;Tian, Yujing;Xiao, Siyun;Xie, Yuan;Du, Zhenguo;Gao, Fangluan
关键词：areca palm; ormycovirus; ORFan; RNA-dependent RNA polymerase
-
A novel Pik allele confers extended resistance to rice blast
作者：Qi, Zhongqiang;Du, Yan;Yu, Junjie;Song, Tianqiao;Pan, Xiayan;Zhang, Rongsheng;Cao, Huijuan;Yu, Mina;Liu, Yongfeng;Qi, Zhongqiang;Zhou, Bo;Liu, Yongfeng;Meng, Xiuli;Lu, Guodong;Meng, Xiuli;Telebanco-Yanoria, Mary Jeanie;Zhou, Bo;Meng, Xiuli;Xu, Ming
关键词：AvrPik; BSA-seq; Oryza rufipogon; Pik-W25
-
Molecular Insights into Red Palm Weevil Resistance Mechanisms of Coconut (Cocos nucifera) Leaves
作者：Liu, Li;Yan, Wei;Liu, Bo;Qin, Weiquan
关键词：red palm weevil; Cocos nucifera; photosynthetic markers; metabolomics; transcriptomics
-
Identification and Biological Characterization of Green Alga on Oil-Tea Camellia Leaves
作者：Cao, Qiulin;Liu, Yanju;Xu, Yufen;Yu, Zhaoyan;Wu, Kunlin;Gong, Han;Yang, Yaodong;Song, Weiwei;Jia, Xiaocheng
关键词：oil-tea Camellia; green alga;
Desmodesmus armatus ; identification; biological characterization -
Widely targeted metabolomics combined with E-tongue and E-nose reveal dynamic changes of tender coconut water in responses to the infection of Ceratocystis paradoxa
作者：Shen, Xiaojun;Niu, Xiaoqing;Yang, Yaodong;Yang, Dejie;Li, Jing;Yu, Fengyu;Sun, Xiwei;Meng, Xiuli;Niu, Xiaoqing;Yang, Dejie;Yu, Fengyu;Meng, Xiuli
关键词：Cocos nucifera L.; Postharvest disease; Liquid endosperm; Flavor; Electronic sensing evaluation
-
First report of Neofusicoccum parvum causing leaf spot of Camellia drupifera in China
作者：Yu, Fengyu;Liu, Xiaoyu;Jia, Xiaocheng;Fu, Dengqiang;Song, Weiwei;Niu, Xiaoqing
关键词：Neofusicoccum parvum; Leaf spot disease; Camellia drupifera
