PxTret1-like Affects the Temperature Adaptability of a Cosmopolitan Pest by Altering Trehalose Tissue Distribution
文献类型： 外文期刊
作者： Zhou, Huiling 1 ; Lei, Gaoke 1 ; Chen, Yanting 1 ; You, Minsheng 1 ; You, Shijun 1 ;
作者机构： 1.Fujian Agr & Forestry Univ, Inst Appl Ecol, State Key Lab Ecol Pest Control Fujian & Taiwan C, Fuzhou 350002, Peoples R China
2.Minist Educ, Joint Int Res Lab Ecol Pest Control, Fuzhou 350002, Peoples R China
3.Fujian Agr & Forestry Univ, Ministerial & Prov Joint Innovat Ctr Safety Prod, Fuzhou 350002, Peoples R China
4.Fujian Acad Agr Sci, Inst Plant Protect, Fuzhou 350013, Peoples R China
5.BGI Shenzhen, BGI Sanya, Sanya 572025, Peoples R China
关键词： Plutella xylostella; CRISPR; Cas9; mutant strain; trehalose; temperature adaptability
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）
ISSN：
年卷期： 2022 年 23 卷 16 期
页码：
收录情况： SCI
摘要： Global warming poses new challenges for insects to adapt to higher temperatures. Trehalose is the main blood sugar in insects and plays an important role in energy metabolism and stress resistance. The transmembrane transport of trehalose mainly depends on the trehalose transporter (TRET1). Plutella xylostella (L.) is a worldwide agricultural pest; however, the effects of the trehalose transport mechanism and trehalose distribution in tissues on the development, reproduction and temperature adaptation of P. xylostella have yet to be reported. In this study, PxTret1-like was cloned and analyzed regarding its expression pattern. It was found that the expression of PxTret1-like was affected by ambient temperature. The knockout mutation of PxTret1-like was generated using a CRISPR/Cas9 system by targeted knockout. The trehalose content and trehalase activity of mutant P. xylostella increased at different developmental stages. The trehalose content increased in the fat body of the fourth-instar P. xylostella, and decreased in the hemolymph, and there was no significant change in glucose in the fat body and hemolymph. Mutant strains of P. xylostella showed a significantly reduced survival rate, fecundity and ability to withstand extreme temperatures. The results showed that PxTret1-like could affect the development, reproduction and temperature adaptability of P. xylostella by regulating the trehalose content in the fat body and hemolymph.
- 相关文献
作者其他论文 更多>>
-
Polygenic adaptation of a cosmopolitan pest to a novel thermal environment
作者：Lei, Gaoke;Huang, Jieling;Zhou, Huiling;Chen, Yanting;Vasseur, Liette;You, Minsheng;You, Shijun;Lei, Gaoke;Huang, Jieling;Zhou, Huiling;Chen, Yanting;Vasseur, Liette;You, Minsheng;You, Shijun;Lei, Gaoke;Huang, Jieling;Zhou, Huiling;Chen, Yanting;You, Minsheng;You, Shijun;Chen, Yanting;Song, Jun;Xie, Xuefeng;You, Shijun;Vasseur, Liette;You, Shijun
关键词：CRISPR/Cas9 system; peripheral genes; Plutella xylostella; regulatory network; temperature adaptability
-
Thermal acclimation uncovers a simple genetic basis of adaptation to high temperature in a cosmopolitan pest
作者：You, Shijun;Lei, Gaoke;Zhou, Huiling;Li, Jianyu;Chen, Shaoping;Huang, Jieling;Vasseur, Liette;Gurr, Geoff M.;You, Minsheng;Chen, Yanting;You, Shijun;Lei, Gaoke;Zhou, Huiling;Li, Jianyu;Chen, Shaoping;Huang, Jieling;Vasseur, Liette;Gurr, Geoff M.;You, Minsheng;Chen, Yanting;You, Shijun;Lei, Gaoke;Zhou, Huiling;Li, Jianyu;Chen, Shaoping;Huang, Jieling;You, Minsheng;Chen, Yanting;Vasseur, Liette;Gurr, Geoff M.
关键词：
-
Comparative analysis of habitat suitability for a crop and its primary insect herbivore: providing insights for crop planting and pest management strategies
作者：Li, Jinyu;Mao, Yi;Jiang, Jia;Li, Kai;Li, Jinyu;Zhang, Bang;Mao, Yi;Jiang, Jia;Li, Kai;You, Shijun;Li, Jinyu;Zhang, Bang;Mao, Yi;Jiang, Jia;Li, Kai;You, Shijun;Li, Jinyu;Zhang, Bang;Mao, Yi;Jiang, Jia;Li, Kai;You, Shijun
关键词：land resource planning; species distribution model; land suitability assessment; tea cultivation; tea green leafhopper
-
A very long-chain fatty acid enzyme gene, PxHacd2 affects the temperature adaptability of a cosmopolitan insect by altering epidermal permeability
作者：Lei, Gaoke;Zhou, Huiling;Chen, Yanting;Vasseur, Liette;Gurr, Geoff M.;You, Minsheng;You, Shijun;Lei, Gaoke;Zhou, Huiling;Chen, Yanting;Vasseur, Liette;Gurr, Geoff M.;You, Minsheng;You, Shijun;Lei, Gaoke;Zhou, Huiling;Chen, Yanting;You, Minsheng;You, Shijun;Chen, Yanting;Vasseur, Liette;Gurr, Geoff M.
关键词：Plutella xylostella; Very long-chain fatty acids; Extreme temperature; CRISPR; Cas9; Water balance
-
Contrasting roles of landscape compositions on shaping functional traits of arthropod community in subtropical vegetable fields
作者：Zhang, Jie;Saqib, Hafiz Sohaib Ahmed;Niu, Dongsheng;Guaman, Karla Giovana Gavilanez;Wang, Ao;Yu, Deyi;You, Minsheng;Pozsgai, Gabor;You, Shijun;Zhang, Jie;Yu, Deyi;Zhang, Jie;Niu, Dongsheng;Wang, Ao;You, Minsheng;Saqib, Hafiz Sohaib Ahmed;Niu, Dongsheng;You, Minsheng;Pozsgai, Gabor;You, Shijun
关键词：Habitat transformation; Biological pest control; Functional diversity; Trait composition; Scale-dependent; RLQ analysis
-
Environmental heterogeneity drives population genetic divergence of a key agricultural pest, Empoasca onukii
作者：Li, Jinyu;Shi, Longqing;Chen, Wei;Mao, Yi;Vasseur, Liette;Gurr, Geoff;You, Minsheng;You, Shijun;Li, Jinyu;Mao, Yi;Li, Jinyu;Shi, Longqing;Chen, Wei;Mao, Yi;Vasseur, Liette;Gurr, Geoff;You, Minsheng;You, Shijun;Li, Jinyu;Shi, Longqing;Chen, Wei;Mao, Yi;You, Minsheng;You, Shijun;Shi, Longqing;Vasseur, Liette;Gurr, Geoff
关键词：climate change; adaptation; landscape genetics; evolution; population divergence
-
Infection of Metarhizium anisopliae Ma6 and defense responses of host Phyllotreta striolata adults
作者：Li, Jianyu;Chen, Yanting;He, Yuechao;Zheng, Lizhen;Shi, Mengzhu;Fu, Jianwei;Shi, Mengzhu
关键词：defense response; infection; Metarhizium anisopliae; Phyllotreta striolata; scanning electron microscopy
