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PxTret1-like Affects the Temperature Adaptability of a Cosmopolitan Pest by Altering Trehalose Tissue Distribution

文献类型： 外文期刊

作者： Zhou, Huiling 1 ; Lei, Gaoke 1 ; Chen, Yanting 1 ; You, Minsheng 1 ; You, Shijun 1 ;

作者机构： 1.Fujian Agr & Forestry Univ, Inst Appl Ecol, State Key Lab Ecol Pest Control Fujian & Taiwan C, Fuzhou 350002, Peoples R China

2.Minist Educ, Joint Int Res Lab Ecol Pest Control, Fuzhou 350002, Peoples R China

3.Fujian Agr & Forestry Univ, Ministerial & Prov Joint Innovat Ctr Safety Prod, Fuzhou 350002, Peoples R China

4.Fujian Acad Agr Sci, Inst Plant Protect, Fuzhou 350013, Peoples R China

5.BGI Shenzhen, BGI Sanya, Sanya 572025, Peoples R China

关键词： Plutella xylostella; CRISPR; Cas9; mutant strain; trehalose; temperature adaptability

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 16 期

页码：

收录情况： SCI

摘要： Global warming poses new challenges for insects to adapt to higher temperatures. Trehalose is the main blood sugar in insects and plays an important role in energy metabolism and stress resistance. The transmembrane transport of trehalose mainly depends on the trehalose transporter (TRET1). Plutella xylostella (L.) is a worldwide agricultural pest; however, the effects of the trehalose transport mechanism and trehalose distribution in tissues on the development, reproduction and temperature adaptation of P. xylostella have yet to be reported. In this study, PxTret1-like was cloned and analyzed regarding its expression pattern. It was found that the expression of PxTret1-like was affected by ambient temperature. The knockout mutation of PxTret1-like was generated using a CRISPR/Cas9 system by targeted knockout. The trehalose content and trehalase activity of mutant P. xylostella increased at different developmental stages. The trehalose content increased in the fat body of the fourth-instar P. xylostella, and decreased in the hemolymph, and there was no significant change in glucose in the fat body and hemolymph. Mutant strains of P. xylostella showed a significantly reduced survival rate, fecundity and ability to withstand extreme temperatures. The results showed that PxTret1-like could affect the development, reproduction and temperature adaptability of P. xylostella by regulating the trehalose content in the fat body and hemolymph.

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