文献类型： 外文期刊

作者： Ye, Xiu-Xian ¹ ; Chen, Yi-Quan ² ; Wu, Jian-She ¹ ; Zhong, Huai-Qin ¹ ; Lin, Bing ¹ ; Huang, Min-Ling ¹ ; Fan, Rong-Hui ¹ ;

作者机构： 1.Fujian Acad Agr Sci, Inst Crop Sci, Fuzhou, Fujian, Peoples R China

2.Fujian Acad Agr Sci, Inst Agr Engn Technol, Fuzhou, Fujian, Peoples R China

关键词： Anoectochilus roxburghii (Wall; ) Lindl; Chlorina leaf; Chlorophyll; Anthocyanin; Transcriptome

期刊名称：BIOCHEMICAL GENETICS （ 影响因子：2.4； 五年影响因子：2.2 ）

ISSN： 0006-2928

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Anoectochilus roxburghii (Wall.) Lindl is a perennial herb of the Orchidaceae family; a yellow-green mutant and a yellow mutant were obtained from the wild type, thereby providing good material for the study of leaf color variation. Pigment content analysis revealed that chlorophyll, carotenoids, and anthocyanin were lower in the yellow-green and yellow mutants than in the wild type. Transcriptome analysis of the yellow mutant and wild type revealed that 78,712 unigenes were obtained, and 599 differentially expressed genes (120 upregulated and 479 downregulated) were identified. Using the Kyoto Encyclopedia of Genes and Genomes pathway analysis, candidate genes involved in the anthocyanin biosynthetic pathway (five unigenes) and the chlorophyll metabolic pathway (two unigenes) were identified. Meanwhile, the low expression of the chlorophyll and anthocyanin biosynthetic genes resulted in the absence of chlorophylls and anthocyanins in the yellow mutant. This study provides a basis for similar research in other closely related species.