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Multi-omics analysis reveals the roles of purple acid phosphatases in organic phosphorus utilization by the tropical legume Stylosanthes guianensis

文献类型： 外文期刊

作者： Luo, Jiajia 1 ; Chen, Zhijian 1 ; Huang, Rui 1 ; Wu, Yuanhang 3 ; Liu, Chun 1 ; Cai, Zeping 3 ; Dong, Rongshu 1 ; Arango, Jacobo 4 ; Rao, Idupulapati Madhusudana 4 ; Schultze-Kraft, Rainer 4 ; Liu, Guodao 1 ; Liu, Pandao 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Haikou 571101, Peoples R China

2.Chinese Acad Trop Agr Sci, Natl Key Lab Trop Crop Breeding, Haikou 571101, Peoples R China

3.Hainan Univ, Coll Trop Crops & Coll Forestry, Haikou 570228, Peoples R China

4.Alliance Biovers Int & Int Ctr Trop Agr, Cali 763537, Colombia

关键词： low phosphate tolerance; purple acid phosphatase; phosphorus recycling; multi-omics; tropical legume; Stylosanthes guianensis

期刊名称：PLANT JOURNAL （ 影响因子：7.2； 五年影响因子：7.9 ）

ISSN： 0960-7412

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Stylo (Stylosanthes guianensis) is a tropical legume known for its exceptional tolerance to low phosphate (Pi), a trait believed to be linked to its high acid phosphatase (APase) activity. Previous studies have observed genotypic variations in APase activity in stylo; however, the gene encoding the crucial APase responsible for this variation remains unidentified. In this study, transcriptomic and proteomic analyses were employed to identify eight Pi starvation-inducible (PSI) APases belonging to the purple APase (PAP) family in the roots of stylo and seven in the leaves. Among these PSI-PAPs, SgPAP7 exhibited a significantly positive correlation in its expression levels with the activities of both internal APase and root-associated APase across 20 stylo genotypes under low-Pi conditions. Furthermore, the recombinant SgPAP7 displayed high catalytic activity toward adenosine 5 ' -diphosphate (ADP) and phosphoenolpyruvate (PEP) in vitro. Overexpression (OE) of SgPAP7 in Arabidopsis facilitated exogenous organic phosphorus utilization. Moreover, SgPAP7 OE lines showed lower shoot ADP and PEP levels than the wild type, implying that SgPAP7 is involved in the catabolism and recycling of endogenous ADP and PEP, which could be beneficial for plant growth in low-Pi soils. In conclusion, SgPAP7 is a key gene with a major role in stylo adaptation to low-Pi conditions by facilitating the utilization of both exogenous and endogenous organic phosphorus sources. It may also function as a PEP phosphatase involved in a glycolytic bypass pathway that minimizes the need for adenylates and Pi. Thus, SgPAP7 could be a promising target for improving tolerance of crops to low-Pi availability.

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