The novel Na+/H+ antiporter gene SpNHX1 from Sesuvium portulacastrum confers enhanced salt tolerance to transgenic yeast
文献类型： 外文期刊
作者： Zhou, Yang 1 ; Yang, Chenglong 1 ; Hu, Yanping 3 ; Yin, Xiaochang 2 ; Li, Ruimei 1 ; Fu, Shaoping 1 ; Zhu, Baibi 3 ; Guo, 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Key Lab Biol & Genet Resources Trop Crops, Minist Agr, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China
2.Hainan Univ, Hainan Key Lab Biotechnol Salt Tolerant Corps, Inst Trop Agr & Forestry, Haikou 570228, Hainan, Peoples R China
3.Hainan Acad Agr Sci, Inst Vegetables, Haikou 571100, Hainan, Peoples R China
关键词： Sesuvium portulacastrum; Na+/H+ antiporter; Salt tolerance; Transgenic yeast
期刊名称：ACTA PHYSIOLOGIAE PLANTARUM （ 影响因子：2.354； 五年影响因子：2.711 ）
ISSN： 0137-5881
年卷期： 2018 年 40 卷 3 期
页码：
收录情况： SCI
摘要： The plant Na+/H+ antiporter (NHX) plays a significant role in cellular ion homeostasis. Herein, an Na+/H+ antiporter gene, SpNHX1 from the Sesuvium portulacastrum plant, was obtained by homology-cloning method. The SpNHX1 gene contains 2113 bp with an open-reading frame encoding a polypeptide of 554 amino acid residues with an estimated molecular mass of 61.27 kDa and an isoelectric point of 7.24. The amino acid sequences of SpNHX1 shared a high similarity with Arabidopsis thaliana AtNHX1 (75.81%), AtNHX2 (77.12%) and Oryza sativa OsNHX1 (73.10%). SpNHX1 contains an amiloride-binding region (FFIYLLPPI), which is a highly-conserved domain in plant Na+/H+ antiporters. RT-PCR (reverse transcriptional PCR) analysis showed that the SpNHX1 gene had a high expression level in roots compared to other tissues under normal conditions. The qRT-PCR (quantitative real-time PCR) results indicated that NaCl treatment induced the expression of SpNHX1 gene in the roots, but its transcriptional levels were not influenced by the ABA, PEG, H2O2, heat (42 degrees C) or cold (4 degrees C) stresses. The growth of yeast cells expressing SpNHX1 was better than the non-transgenic control cells in the presence of 30 mM NaCl, 0.4 M KCl, or 0.5 mM LiCl. Furthermore, transgenic yeast cells with SpNHX1 accumulated more Na+ relative to control cells under salinity stress. These results suggested that SpNHX1 was a key determinant in the salt-stress response in Sesuvium portulacastrum.
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