Whole Genomic EST-SSR Development Based on High-Throughput Transcript Sequencing in Proso Millet (Panicum miliaceum)
文献类型： 外文期刊
作者： Jiang, Yanmiao 1 ; Li, Haiquan 1 ; Zhang, Jianan 1 ; Xiang, Jinying 1 ; Cheng, Ruhong 1 ; Liu, Guoqing 1 ;
作者机构： 1.Hebei Acad Agr & Forestry Sci, Inst Millet Crops, Shijiazhuang 050035, Hebei, Peoples R China
2.Key Lab Minor Crops Hebei, Shijiazhuang 050035, Hebei, Peoples R China
关键词： Proso millet; High-throughput sequencing; SSR development; Characterization
期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）
ISSN： 1560-8530
年卷期： 2018 年 20 卷 3 期
页码：
收录情况： SCI
摘要： Proso millet (Panicum miliaceum L.) with short growing period, is highly suited to drought and semi-arid climate, and thus possesses great potential for research. In this study, 8139 Expressed Sequence Tags-simple sequence repeat (EST-SSR) makers were developed from the transcript sequencing of Proso millet cultivar Yanshu 5. Tri-type was the major type, accounting for 83.65% of all the found SSR types, followed by di-type accounting for 11.1%. CCG/CGG was the most ample repeat-motif types in Proso millet. Twenty four primer pairs of these markers were synthesized for empirical validation and 21 primers obtained targeted fragments by PCR amplification. Three of the 21 markers were polymorphic among 20 Chinese Proso millet landraces which suggested that these EST-SSR markers can be used for genetic studies. Markers developed in this work can be used for agronomic trait location, linkage map construction and molecular breeding in Proso millet in the future. (c) 2018 Friends Science Publishers.
- 相关文献
作者其他论文 更多>>
-
Genetic linkage map construction and QTL analysis for plant height in proso millet (Panicum miliaceum L.)
作者：Jiang, Yanmiao;Dong, Li;Li, Haiquan;Liu, Yanan;Liu, Guoqing;Wang, Xindong;Jiang, Yanmiao;Dong, Li;Li, Haiquan;Liu, Yanan;Liu, Guoqing
关键词：
-
Utilizing machine learning and bioinformatics analysis to identify drought-responsive genes affecting yield in foxtail millet
作者：Zhu, Chunhui;Niu, Xingfang;Wang, Litao;Zhao, Ling;Zhao, Shaoxing;Li, Lin;Liu, Jiaxin;Zhang, Ting;Cheng, Ruhong;Shi, Zhigang;Zhang, Haoshan;Wang, Genping;Niu, Xingfang;Wang, Litao;Gao, Hui;Liu, Jiaxin
关键词：Drought stress; Haplotype; Machine learning; Foxtail millet
-
Identification of Putative Quantitative Trait Loci for Improved Seed Oil Quality in Peanuts
作者：Hu, Pengju;Zhang, Jianan;Song, Yahui;Zhao, Xing;Jin, Xinxin;Su, Qiao;Yang, Yongqing;Wang, Jin
关键词：peanut; oil quality; fatty acids; QTL; epistatic effects
-
Genomic variation in weedy and cultivated broomcorn millet accessions uncovers the genetic architecture of agronomic traits
作者：Lu, Qiong;Zhao, Hainan;Zhang, Zhengquan;Bai, Yuhe;Zhao, Haiming;Zheng, Yunxiao;Zhao, Haiyue;Gong, Huihui;Chen, Lingwei;Deng, Xizhen;Hong, Xiangde;Liu, Tianxiang;Li, Baichuan;Bai, Yan;Xin, Beibei;Chen, Jian;E., Lizhu;Lai, Jinsheng;Song, Weibin;Zhao, Hainan;Lai, Jinsheng;Song, Weibin;Liu, Guoqing;Li, Haiquan;Liu, Minxuan;Lu, Ping;Wen, Feng;Wang, Lun;Li, Zhijiang;Li, Hai;Zhang, Like;Ma, Wenhui;Liu, Chunqing;Bai, Yan;Lai, Jinsheng;Song, Weibin;Lai, Jinsheng;Song, Weibin
关键词：
-
Development of SNP marker panels for genotyping by target sequencing (GBTS) and its application in soybean
作者：Yang, Qing;Shi, Xiaolei;Qin, Jun;Zhang, Mengchen;Yang, Chunyan;Yan, Long;Zhang, Jianan;Chen, Lei;Song, Qijian
关键词：Soybean breeding; Marker-assisted selection (MAS); SNP marker panel; Genotyping by target sequencing (GBTS); Germplasm evaluation; QTL identification
-
Haplotype variation and KASP markers for SiPSY1 - A key gene controlling yellow kernel pigmentation in foxtail millet
作者：Zuo, Rongjun;Zhang, Yanyan;Wang, Chunfang;Zhi, Hui;Zhang, Linlin;Tang, Sha;Jia, Guanqing;Diao, Xianmin;Yang, Yanbing;Guan, Yanan;Li, Shunguo;Cheng, Ruhong;Diao, Xianmin;Zuo, Rongjun;Wang, Chunfang;Shang, Zhonglin;Diao, Xianmin
关键词：Carotenoids; Lutein; Zeaxanthin; Marker assisted selection; Setaria italica
-
Pangenome analysis reveals genomic variations associated with domestication traits in broomcorn millet
作者：Chen, Jinfeng;Liu, Minxuan;He, Qiang;Zhang, Wei;Gao, Yuanzhu;Jia, Guanqing;Lu, Ping;Zhi, Hui;Tang, Sha;Diao, Xianmin;Chen, Jinfeng;Liu, Yang;Guo, Wenlei;Chen, Weiyao;Zhang, Xiaoming;Guo, Wenlei;Chen, Weiyao;Wang, Yongqiang;Liao, Yi;Dong, Kongjun;Ren, Ruiyu;Yang, Tianyu;Zhang, Liyuan;Qi, Mingyu;Li, Zhiguang;Zhao, Min;Wang, Haigang;Wang, Junjie;Qiao, Zhijun;Li, Haiquan;Jiang, Yanmiao;Liu, Guoqing;Song, Xiaoqiang;Deng, Yarui;Li, Hai;Yan, Feng;Dong, Yang;Li, Qingquan;Li, Tao;Yang, Wenyao;Cui, Jianghui;Wang, Hongru;Zhou, Yongfeng
关键词：
