RNA interference of glutamate-gated chloride channel decreases abamectin susceptibility in Bemisia tabaci
文献类型： 外文期刊
作者： Wei, Peiling 1 ; Che, Wunan 3 ; Wang, Jinda 4 ; Xiao, Da 1 ; Wang, Ran 1 ; Luo, Chen 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China
2.China Agr Univ, Dept Entomol, Beijing 100193, Peoples R China
3.Shenyang Agr Univ, Dept Pesticide Sci, Shenyang 110866, Liaoning, Peoples R China
4.Fujian Agr & Forestry Univ, Natl Engn Res Ctr Sugarcane, Fuzhou 350002, Fujian, Peoples R China
关键词： Bemisia tabaci; Glutamate-gated chloride channel; Double-stranded RNA; Abamectin; mRNA expression; Mortality
期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）
ISSN： 0048-3575
年卷期： 2018 年 145 卷
页码：
收录情况： SCI
摘要： The Bemisia tabaci (Gennadius) cryptic species complex comprises very destructive insect pests of agricultural crops worldwide and has been found to be resistant to various insecticides in China. Abamectin is one of the most widely used insecticides for insect pest control and the glutamate-gated chloride channel (GIuCl) in insects was presumed to be the main target site of abamectin. In this study, a 1353 bp full-length cDNA encoding GIuCI (named BtGluCl, GenBank ID: MF673854) was cloned and characterized from B. tabaci. BtGluCl encodes 450 amino acids, which shares 71-81% identity with other insect GluCl isoforms. Spatial and temporal expression revealed BtGluCl was highly expressed in the 4th nymphal instar and adult head, and the least expressed in the 1st nymphal instar and adult leg. Dietary ingestion of dsBtGluCl significantly reduced the mRNA level of BtGluCl in the treated adults by 62.9% and greatly decreased abamectin-induced mortality. Thus, our results could be conducive to further understanding the mechanisms of resistance to abamectin in arthropods.
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