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Characterization of a sterile dwarf mutant and the cloning of zeaxanthin epoxidase in Asian cotton (Gossypium arboreum L.)

文献类型： 外文期刊

作者： Wu, Chuntai 1 ; Zhou, Baoliang 2 ;

作者机构： 1.CATAS, Minist Agr, Rubber Res Inst, Key Lab Biol & Genet Resources Rubber Tree, Danzhou 571737, Hainan, Peoples R China

2.Nanjing Agr Univ, Cotton Res Inst, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China

关键词： Gossypium arboretum; Sd(a); Radiation mutation; Deletion; Abscisic acid; Zeaxanthin epoxidase (ZEP) gene

期刊名称：PLANT GROWTH REGULATION （ 影响因子：3.412； 五年影响因子：3.691 ）

ISSN： 0167-6903

年卷期： 2018 年 85 卷 1 期

页码：

收录情况： SCI

摘要： To document radiation damage incurred by nuclear DNA and understand the molecular mechanisms that control plant height, a sterile dwarf mutant (sd (a) ) derived from the Gossypium arboreum treated with Co-60 gamma-rays was characterized. A sequence analysis revealed that sd (a) included a partial deletion and a point mutation. The physiological changes and responses of the sd (a) mutant to exogenous hormones showed that the mutant's chlorophyll contents, net photosynthetic rate, and abscisic acid levels were significantly reduced compared to those of wild-type. Using the sequence of a differentially expressed sequence tag encoding a zeaxanthin epoxidase (ZEP), a novel etiolation-associated gene, full-length cDNA was rapidly cloned using rapid amplification of cDNA ends. The full-length cDNA of this gene is 2414 bp, with a 2004-bp open reading frame encoding a putative ZEP of 667 amino acid residues that has a 73.421-kDa theoretical molecular mass and a 6.67 isoelectric point. The genomic sequence of GaZEP is 5233 bp, comprising 15 exons and 14 introns. The GaZEP promoter contains numerous cis-regulatory elements that are responsive to light, hormones, and stress. Real-time quantitative PCR indicated that GaZEP expression in the mutant was downregulated. The expression levels of GaZEP in roots and leaves were positively correlated with the root hair lengths and abscisic acid levels, respectively. Thus, in photosynthetic tissues, GaZEP mRNA levels might be regulated coordinately with variations in the abscisic acid levels. These results may assist in the further functional dissection of ZEP and in elucidating the molecular mechanism underlying sterile dwarfism in cotton.

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