A RAD-Based Genetic Map for Anchoring Scaffold Sequences and Identifying QTLs in Bitter Gourd (Momordica charantia)
文献类型： 外文期刊
作者： Cui, Junjie 2 ; Luo, Shaobo 3 ; Niu, Yu 4 ; Huang, Rukui 5 ; Wen, Qingfang 6 ; Su, Jianwen 7 ; Miao, Nansheng 8 ; He, Wei 1 ;
作者机构： 1.South China Agr Univ, Coll Hort, Guangzhou, Guangdong, Peoples R China
2.South China Agr Univ, Coll Hort, Minist Agr, Key Lab Biol & Germplasm Enhancement Hort Crops S, Guangzhou, Guangdong, Peoples R China
3.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangzhou, Guangdong, Peoples R China
4.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Danzhou, Peoples R China
5.Guangxi Acad Agr Sci, Vegetable Res Inst, Nanning, Peoples R China
6.Fujian Acad Agr Sci, Crops Res Inst, Fuzhou, Fujian, Peoples R China
7.Hunan Vegetable Res Inst, Changsha, Hunan, Peoples R China
8.Jiangxi Acad Agr Sci, Inst Vegetables & Flowers, Nanchang, Jiangxi, Peoples R China
9.Beijing Genom Inst, Shenzhen, Peoples R China
关键词： bitter gourd; restriction site associated DNA (RAD); genetic map; genome assembly; genetic location
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）
ISSN： 1664-462X
年卷期： 2018 年 9 卷
页码：
收录情况： SCI
摘要： Genetic mapping is a basic tool necessary for anchoring assembled scaffold sequences and for identifying QTLs controlling important traits. Though bitter gourd (Momordica charantia) is both consumed and used as a medicinal, research on its genomics and genetic mapping is severely limited. Here, we report the construction of a restriction site associated DNA (RAD)-based genetic map for bitter gourd using an F-2 mapping population comprising 423 individuals derived from two cultivated inbred lines, the gynoecious line 'K44' and the monoecious line 'Dali-11.' This map comprised 1,009 SNP markers and spanned a total genetic distance of 2,203.95 cM across the 11 linkage groups. It anchored a total of 113 assembled scaffolds that covered about 251.32 Mb (85.48%) of the 294.01 Mb assembled genome. In addition, three horticulturally important traits including sex expression, fruit epidermal structure, and immature fruit color were evaluated using a combination of qualitative and quantitative data. As a result, we identified three QTL/gene loci responsible for these traits in three environments. The QTL/gene gy/fffn/ffn, controlling sex expression involved in gynoecy, first female flower node, and female flower number was detected in the reported region. Particularly, two QTLs/genes, Fwa/Wr and w, were found to be responsible for fruit epidermal structure and white immature fruit color, respectively. This RAD-based genetic map promotes the assembly of the bitter gourd genome and the identified genetic loci will accelerate the cloning of relevant genes in the future.
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