CsATAF1 Positively Regulates Drought Stress Tolerance by an ABA-Dependent Pathway and by Promoting ROS Scavenging in Cucumber
文献类型： 外文期刊
作者： Wang, Jinfang 1 ; Zhang, Lei 1 ; Cao, Yunyun 1 ; Qi, Chuandong 1 ; Li, Shuangtao 1 ; Liu, Lun 1 ; Wang, Gongle 1 ; Mao, A 1 ;
作者机构： 1.China Agr Univ, Coll Hort, Beijing Key Lab Growth & Dev Regulat Protected Ve, Beijing 100193, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Beijing Key Lab Vegetable Germplasm Improvement, Beijing 100097, Peoples R C
关键词： ABA-dependent pathway; Cucumis sativus L; Drought stress; NAC transcription factor; ROS scavenging
期刊名称：PLANT AND CELL PHYSIOLOGY （ 影响因子：4.927； 五年影响因子：5.516 ）
ISSN： 0032-0781
年卷期： 2018 年 59 卷 5 期
页码：
收录情况： SCI
摘要： The NAC transcription factors play vital roles in responding to drought stress in plants; however, the molecular mechanisms remain largely unknown in cucumber. Suppression of CsATAF1 via RNA interference (RNAi) weakened drought stress tolerance in cucumber due to a higher water loss rate in leaves, a higher level of hydrogen peroxide (H2O2) and superoxide radicals (O-2 center dot-), increased malondialdehyde (MDA) content, lower F-v/F-m ratios and lower antioxidant enzyme activity. The analysis of root length and stomatal apertures showed that CsATAF1-RNAi cucumber plants were less responsive to ABA. In contrast, CsATAF1-overexpression (OE) plants showed increased drought stress tolerance and sensitivity to ABA. Quantitative PCR (qPCR) analysis showed that expression of several stress-responsive genes was significantly up-regulated in CsATAF1-OE transformants and down-regulated in CsATAF1-RNAi transformants. CsAB15, CsCu-ZnSOD and CsDREB2C were verified as direct target genes of CsATAF1. Yeast one-hybrid analysis and electrophoretic mobility shift assay (EMSA) further substantiated that CsATAF1 bound to the promoters of CsAB15, CsCu-ZnSOD and CsDREB2C. Transient expression in tobacco leaves and cucumber protoplasts showed that CsATAF1 directly up-regulated the expression of CsAB15, CsCu-ZnSOD and CsDREB2C. Our results demonstrated that CsATAF1 functioned as a positive regulator in response to drought stress by an ABA-dependent pathway and decreasing reactive oxygen species (ROS) accumulation in cucumber.
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