CsATAF1 Positively Regulates Drought Stress Tolerance by an ABA-Dependent Pathway and by Promoting ROS Scavenging in Cucumber
文献类型： 外文期刊
作者： Wang, Jinfang 1 ; Zhang, Lei 1 ; Cao, Yunyun 1 ; Qi, Chuandong 1 ; Li, Shuangtao 1 ; Liu, Lun 1 ; Wang, Gongle 1 ; Mao, A 1 ;
作者机构： 1.China Agr Univ, Coll Hort, Beijing Key Lab Growth & Dev Regulat Protected Ve, Beijing 100193, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Beijing Key Lab Vegetable Germplasm Improvement, Beijing 100097, Peoples R C
关键词： ABA-dependent pathway; Cucumis sativus L; Drought stress; NAC transcription factor; ROS scavenging
期刊名称：PLANT AND CELL PHYSIOLOGY （ 影响因子：4.927； 五年影响因子：5.516 ）
ISSN： 0032-0781
年卷期： 2018 年 59 卷 5 期
页码：
收录情况： SCI
摘要： The NAC transcription factors play vital roles in responding to drought stress in plants; however, the molecular mechanisms remain largely unknown in cucumber. Suppression of CsATAF1 via RNA interference (RNAi) weakened drought stress tolerance in cucumber due to a higher water loss rate in leaves, a higher level of hydrogen peroxide (H2O2) and superoxide radicals (O-2 center dot-), increased malondialdehyde (MDA) content, lower F-v/F-m ratios and lower antioxidant enzyme activity. The analysis of root length and stomatal apertures showed that CsATAF1-RNAi cucumber plants were less responsive to ABA. In contrast, CsATAF1-overexpression (OE) plants showed increased drought stress tolerance and sensitivity to ABA. Quantitative PCR (qPCR) analysis showed that expression of several stress-responsive genes was significantly up-regulated in CsATAF1-OE transformants and down-regulated in CsATAF1-RNAi transformants. CsAB15, CsCu-ZnSOD and CsDREB2C were verified as direct target genes of CsATAF1. Yeast one-hybrid analysis and electrophoretic mobility shift assay (EMSA) further substantiated that CsATAF1 bound to the promoters of CsAB15, CsCu-ZnSOD and CsDREB2C. Transient expression in tobacco leaves and cucumber protoplasts showed that CsATAF1 directly up-regulated the expression of CsAB15, CsCu-ZnSOD and CsDREB2C. Our results demonstrated that CsATAF1 functioned as a positive regulator in response to drought stress by an ABA-dependent pathway and decreasing reactive oxygen species (ROS) accumulation in cucumber.
- 相关文献
作者其他论文 更多>>
-
SlWRKY37 targets SlLEA2 and SlABI5-like7 to regulate seed germination vigor in tomato
作者：Wang, Zhirong;Zhang, Jialong;Deng, Qilin;Guo, Yang-Dong;Zhang, Jialong;Deng, Qilin;Zhang, Yumeng;Pei, Manying;Zhao, Yinling;Zhang, Haijun;Zhang, Haijun;Zhang, Haijun;Zhang, Haijun;Wang, Zhirong
关键词：Phosphorylation; Seed dormancy; SlABI5-like7; Solanum lycopersicum; Transcriptive activation
-
Target Analysis of Polychlorinated Naphthalenes and Nontarget Screening of Organic Chemicals in Bovine Milk, Infant Formula, and Adult Milk Powder by High-Resolution Mass Spectrometry
作者：Qi, Ziyuan;Zhang, Zherui;Zheng, Minghui;Liu, Guorui;Qi, Ziyuan;Zhang, Zherui;Jin, Rong;Zheng, Minghui;Lin, Bingcheng;Liu, Yahui;Liu, Guorui;Qi, Ziyuan;Zhang, Zherui;Zheng, Minghui;Liu, Guorui;Zhang, Lei;Li, Jingguang;Wu, Yongning;Li, Cheng
关键词：polychlorinated naphthalene; risk assessment; possible source; high-resolutionmass spectrometry; organic pollutants
-
Comprehensive Profiling of Alternative Splicing and Alternative Polyadenylation during Fruit Ripening in Watermelon (Citrullus lanatus)
作者：Yu, Yongtao;Ren, Yi;Zhang, Jie;Li, Maoying;Tian, Shouwei;Wang, Jinfang;Liao, Shengjin;Gong, Guoyi;Zhang, Haiying;Guo, Shaogui;Liufu, Yuxiang
关键词：watermelon; alternative splicing; alternative polyadenylation; lncRNA; fruit ripening
-
Insight into growth and wood properties based on QTL and eQTL mapping in Populus deltoides 'Danhong' x Populus simonii 'Tongliao1'
作者：Zhang, Lei;Ge, Xiaolan;Du, Jiujun;Wen, Shuangshuang;Xiang, Xiaodong;Du, Changjian;Zhou, Xinglu;Hu, Jianjun;Zhang, Lei;Hu, Jianjun;Lu, Dongye
关键词：Populus; QTL; eQTL; Xylem development; Co-expression network; Calmodulin
-
Organic fertilizer and irrigation water are the primary sources of microplastics in the facility soil, Beijing
作者：Guo, Sen;Zhang, Jiajia;Liu, Jiawei;Wang, Xuexia;Zhao, Meng;Chen, Yanhua;Guo, Sen;Liu, Jiawei;Zhang, Baogui;Guo, Ning;Zhang, Lei;Wang, Shengtao
关键词：Microplastic; Facility agriculture soil; Distribution characteristic; Ecological risk; Potential source
-
Complete chloroplast genomes of three sand-fixing Salix shrubs from Northwest China: comparative and phylogenetic analysis and interspecific identification
作者：Lu, Dongye;Huang, Haiguang;Zhang, Guosheng;Lu, Dongye;Huang, Haiguang;Zhang, Lei;Hao, Lei
关键词：Salix gordejevii; Salix cheilophila; Salix psammophila; Chloroplast genome; Comparative analysis; Phylogenetic relationship
-
ClSnRK2.3 negatively regulates watermelon fruit ripening and sugar accumulation
作者：Wang, Jinfang;Wang, Yanping;Yu, Yongtao;Zhang, Jie;Ren, Yi;Tian, Shouwei;Li, Maoying;Liao, Shengjin;Guo, Shaogui;Gong, Guoyi;Zhang, Haiying;Xu, Yong
关键词：fruit ripening; SnRK2; 3; sucrose; watermelon
