QTL-Seq Identified a Major QTL for Grain Length and Weight in Rice Using Near Isogenic F-2 Population
文献类型： 外文期刊
作者： Qin Yaobin 1 ; Cheng Peng 2 ; Cheng Yichen 1 ; Feng Yue 1 ; Huang Derun 1 ; Huang Tingxu 3 ; Song Xianjun 2 ; Ying Jiezhe 1 ;
作者机构： 1.China Natl Rice Res Inst, Chinese Natl Ctr Rice Improvement, State Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China
2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
3.Fujian Acad Agr Sci, Fuzhou Natl Subctr Rice Improvement, Inst Rice Res, Fuzhou 350018, Fujian, Peoples R China
关键词： grain length; grain weight; QTL-seq; quantitative trait locus; near-isogenic line; rice; single nucleotide polymorphism; recombinant inbred line; qTGW5.3
期刊名称：RICE SCIENCE （ 影响因子：3.333； 五年影响因子：4.226 ）
ISSN： 1672-6308
年卷期： 2018 年 25 卷 3 期
页码：
收录情况： SCI
摘要： Mapping and isolation of quantitative trait loci (QTLs) or genes controlling grain size or weight is very important to uncover the molecular mechanisms of seed development and crop breeding. To identify the QTLs controlling grain size and weight, we developed a near isogenic line F-2 (NIL-F-2) population, which was derived from a residual heterozygous plant in an F-7 generation of recombinant inbred line (RIL). With the completion of more than 30x whole genome re-sequencing of the parents, two DNA bulks for large and small grains, a total of 58.94 Gb clean nucleotide data were generated. A total of 455 262 single nucleotide polymorphisms (SNPs) between the parents were identified to perform bulked QTL-seq. A candidate genomic region containing SNPs strongly associated with grain length and weight was identified from 15 to 20 Mb on chromosome 5. We designated the major QTL in the candidate region as qTGW5.3. Then, qTGW5.3 was further validated with PCR-based conventional QTL mapping method through developing simple sequence repeat and Insertion/Deletion markers in the F-2 population. Furthermore, recombinants and the progeny tests delimited the candidate region of qTGW5.3 to 1.13 Mb, flanked by HX5009 (15.15 Mb) and HX5003 (16.28 Mb). A set of NILs, selected from the F-2 population, was developed to evaluate the genetic effect of qTGW5.3. Significant QTL effects were detected on grain length, grain width and 1000-grain weight of H12-29 allele with 1.14 mm, -0.11 mm and 3.11 g, which explained 99.64%, 95.51% and 97.32% of the phenotypic variations, respectively.
- 相关文献
