Development of a TaqMan-based real-time PCR assay for the rapid and specific detection of pigeon torque teno virus
文献类型： 外文期刊
作者： Wan, Chunhe 1 ; Chen, Cuiteng 1 ; Cheng, Longfei 1 ; Fu, Guanghua 1 ; Shi, Shaohua 1 ; Fu, Qiuling 2 ; Liu, Rongchang; 1 ;
作者机构： 1.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fujian Prov Key Lab Avian Dis Control & Prevent, Fujian Anim Dis Control Technol Dev Ctr, Fuzhou 350013, Fujian, Peoples R China
2.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fujian Prov Key Lab Avian Dis Control & Prevent, Fujian Anim Dis Control Technol Dev Ctr, Fu
关键词： Pigeon torque teno virus; Real-time PCR; Vertical transmission
期刊名称：MOLECULAR AND CELLULAR PROBES （ 影响因子：2.365； 五年影响因子：2.386 ）
ISSN： 0890-8508
年卷期： 2018 年 39 卷
页码：
收录情况： SCI
摘要： Pigeon torque teno virus (PTTV), a recently discovered circular DNA virus. Here, we developed a TaqMan-based real-time PCR for rapid and specific detection of PTTV infections with sensitivity up to 49.3 copies/mu l. Positive signals can be observed by the assay in pigeon embryonated eggs, which indicted that PTTV can be transmitted vertically. Our findings play important implications for a better understanding the transmission of torque teno virus in pigeons.
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