Rapid detection of goose hemorrhagic polyomavirus using TaqMan quantitative real-time PCR
文献类型： 外文期刊
作者： Wan, Chunhe 1 ; Cheng, Longfei 1 ; Fu, Guanghua 1 ; Chen, Cuiteng 1 ; Liu, Rongchang 1 ; Shi, Shaohua 2 ; Chen, Hongme 1 ;
作者机构： 1.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fujian Prov Key Lab Avian Dis Control & Prevent, Fujian Anim Dis Control Technol Dev Ctr, Fuzhou 350013, Fujian, Peoples R China
2.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fujian Prov Key Lab Avian Dis Control & Prevent, Fujian Anim Dis Control Technol Dev Ctr, Fu
关键词： Goose hemorrhagic polyomavirus; TaqMan real-time PCR; Sensitivity; Domestic Jinyun Sheldrakes
期刊名称：MOLECULAR AND CELLULAR PROBES （ 影响因子：2.365； 五年影响因子：2.386 ）
ISSN： 0890-8508
年卷期： 2018 年 39 卷
页码：
收录情况： SCI
摘要： Due to low doses of infection, an efficient and sensitive virus detection method is necessary to detect low amounts of goose hemorrhagic polyomavirus (GHPV). In this study, we have developed a TaqMan real-time PCR (qPCR) specific assay for the detection of GHPV. Specificity assay showed no cross-reactions with other common waterfowl viruses. The standard curve had a linear correlation of 0.997 and efficiency of 99% between the cycle threshold value and the logarithm of the plasmids copy number. The possible lowest detectable concentration was 35.4 copies/mu l; 100 times more sensitive than conventional PCR (detection limit, 3.54 x 10(3) copies/mu l). Domestic Jinyun Sheldrakes ducks and their embryonated eggs were found positive of GHPV infection which provides evidence of possible vertical transmission of GHPV.
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