文献类型： 外文期刊

作者： Qi, N. L. ¹ ; Gong, S. ¹ ; Yang, C. L. ¹ ; Cheng, Z. H. ¹ ; Zhou, W. ¹ ; Li, J. H. ¹ ;

作者机构： 1.Chinese Acad Trop Agr Sci, Agr Prod Proc Res Inst, Zhanjiang 524001, Peoples R China

2.Chinese Acad Trop Agr Sci, Minist Agr Zhanjiang, Ctr Food Qual Supervis & Testing, Zhanjiang 524001, Peoples R China

3.Minist Agr, Key Lab Trop Crop Prod Proc, Zhanjiang 524001, Peoples R China

4.Huazhong Agr Univ, Coll Food Sci & Technol,

关键词： Lactobacillus salivarius; metabolomics; hyper-osmotic stress; quantitative H-1 NMR spectroscopy; principal component analysis

期刊名称：APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH （ 影响因子：0.711； 五年影响因子：0.796 ）

ISSN： 1589-1623

年卷期： 2018 年 16 卷 3 期

页码：

收录情况： SCI

摘要： In their natural habitats and application environments, lactic acid bacteria are often exposed to osmotic changes. At metabolic level, bacteria are able to de novo synthesize or uptake specific solutes from environment to maintain constant turgor pressure. In the work, the H-1 nuclear magnetic resonance (NMR) spectroscopy based on intracellular metabolites profiles of Lactobacillus salivarius under hyper-osmotic growth conditions was characterized. New characteristic peaks of compatible compounds, such as betaine, carnitine, choline and/or their derivatives were detected. Especially, the cytoplasmic concentrations of proline increased dramatically when the osmolarity was raised from about 0.40 to 1.84 osmol/kg by adding 0.8 mol/l NaCl to the medium. Comparative analysis of the metabolites of L. salivarius FDB89 grown under aerobic and anaerobic conditions with NaCl stress revealed an overlap of up-regulated compounds of more than 50%. 2-aminobutyrate, pyruvate, glycine, trimethylamine-N-oxide, and acetylcholine were detected only under anaerobic condition, and 2-oxoglutarate, tyrosine, glutamine and glutamate were detected only under aerobic condition, which suggested oxygen also has a nonnegligible effect on osmotic response in L. salivarius FDB89.