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Silence of ryanodine receptor gene decreases susceptibility to chlorantraniliprole in the oriental armyworm, Mythimna separata Walker

文献类型： 外文期刊

作者： Wang, Jin-Da 1 ; Chen, Li-Fei 1 ; Wang, Ya-Ru 1 ; Fu, Hua-Ying 1 ; Ali, Ahmad 1 ; Xiao, Da 2 ; Wang, Ran 2 ; Gao, San-Ji 1 ;

作者机构： 1.Fujian Agr & Forestry Univ, Natl Engn Res Ctr Sugarcane, Fuzhou 350002, Fujian, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China

关键词： Ryanodine receptor; Mythimna separata; Chlorantraniliprole; RNAi

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）

ISSN： 0048-3575

年卷期： 2018 年 148 卷

页码：

收录情况： SCI

摘要： The ryanodine receptors of insects are the main target sites of diamide insecticides, which show highly selective insecticidal activity relative to toxicity in mammals and provide a novel option for managing lepidopteran pests. The oriental army worm, Mythimna separata (Walker), is a destructive pest of agricultural crops, and great efforts have been undertaken to control this pest including repeated insecticide applications. In this study, full-length cDNA of a ryanodine receptor gene from M. separata (MsRyR) was cloned and characterized. The cDNA of MsRyR had a 15,372 bp open reading frame and encoded 5124 amino acids (GenBank ID: MG712298). MsRyR shares 78-97% identity with RyR isoforms of other insects, and < 50% identities with Homo sapiens RyRs 1-3. Temporal and spatial expression analysis detected MsRyR at all developmental stages and in all tissues. The highest relative levels of MsRyR were detected in the second instar and head. Exposure to chlorantraniliprole after 24 h significantly increased the expression levels of whole body MsRyR mRNA. In addition, dietary ingestion of dsMsRyR significantly reduced the mRNA level of MsRyR and greatly decreased chlorantraniliprole-induced mortality. Our results revealed that the MsRyR could be the molecular target of chlorantraniliprole, and provided the basis for further understanding the resistance mechanism of chlorantraniliprole.

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