Characterization of Haemophilus parasuis Serovar 2 CL120103, a Moderately Virulent Strain in China
文献类型： 外文期刊
作者： Che, Yongliang 1 ; Wang, Longbai 1 ; Wu, Xuemin 1 ; Chen, Rujing 1 ; Wang, Chenyan 1 ; Zhou, Lunjiang 1 ;
作者机构： 1.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou, Fujian, Peoples R China
关键词： Haemophilus parasuis; genome sequencing; comprehensive genetic analysis; virulence-associated gene; LPS biosynthesis; virulence
期刊名称：OPEN LIFE SCIENCES （ 影响因子：0.938； 五年影响因子：1.154 ）
ISSN： 2391-5412
年卷期： 2018 年 13 卷 1 期
页码：
收录情况： SCI
摘要： Haemophilus parasuis is an important bacterium affecting pigs, causing Glasser's disease. To further characterize this species, we determined the complete genomic sequence of H. parasuis CL120103, which was isolated from diseased pigs. The strain H. parasuis CL120103 was identified as serovar 2. The size of the largest scaffold is 2,326,318 bp and contains 145 large contigs, with the N50 contig being 20,573 bp in length. The complete genome of H. parasuis CL120103 is 2,305,354 bp in length with 39.97% GC content and contains 2227 protein-coding genes, 19 ribosomal rRNA operons and 60 tRNA genes. Sequence similarity of the genome of H. parasuis CL120103 to the previously sequenced genome of H. parasuis was up to 96% and query cover to 86%. Annotation of the genome of H. parasuis CL120103 identified a number of genes encoding potential virulence factors. These virulence factors are involved in metabolism, adhesion, secretion and LPS biosynthesis. These related genes pave the way to better understand mechanisms underlying metabolic capabilities. The comprehensive genetic and phylogenetic analysis shows that H. parasuis is closely related to Actinobacillus pleuropneumoniae and provides a foundation for future experimental confirmation of the virulence and pathogen-host interactions in H. parasuis.
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