Transgenic expression of plastidic glutamine synthetase increases nitrogen uptake and yield in wheat
文献类型： 外文期刊
作者： Hu, Mengyun 1 ; Zhao, Xueqiang 2 ; Liu, Qian 1 ; Hong, Xia 2 ; Zhang, Wei 2 ; Zhang, Yingjun 1 ; Sun, Lijing 1 ; Li, Hui; 1 ;
作者机构： 1.Hebei Acad Agr & Forestry Sci, Inst Cereal & Oil Crops, Hebei Lab Crop Genet & Breeding, Shijiazhuang, Hebei, Peoples R China
2.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing, Peoples R China
3.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab P
关键词： wheat; glutamine synthetase; nitrogen use efficiency
期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：9.803； 五年影响因子：9.555 ）
ISSN： 1467-7644
年卷期： 2018 年 16 卷 11 期
页码：
收录情况： SCI
摘要： The plastidic glutamine synthetase isoform (GS2) plays a key role in nitrogen (N) assimilation. We introduced TaGS2- 2Abpro::TaGS2-2Ab, the favourable allele of TaGS2-2A in the winter wheat (Triticum aestivum) variety Ji5265. Transgenic expression of TaGS2-2Ab significantly increased GS2 abundance and GS activity in leaves. Two consecutive field experiments showed the transgenic lines had higher grain yield, spike number, grain number per spike and 1000-grain weight than did the wild type under both low N and high N conditions. Analysis of N use-related traits showed that transgenic expression of TaGS2-2Ab increased root ability to acquire N, N uptake before and after flowering, remobilization of N to grains and N harvest index. Measurement of chlorophyll content and net photosynthesis rate in flag leaves during grain filling stage revealed that the transgenic lines had prolonged leaf functional duration as compared with the wild type. These results suggest that TaGS2 plays important role in N use, and the favourable allele TaGS2-2Ab is valuable in engineering wheat with improved N use efficiency and grain yield.
- 相关文献
作者其他论文 更多>>
-
TaLBD41 interacts with TaNAC2 to regulate nitrogen uptake and metabolism in response to nitrate availability
作者：Jing, Yanfu;Shen, Chuncai;Peng, Lei;Zhao, Xueqiang;Teng, Wan;Tong, Yiping;He, Xue;Jing, Yanfu;Peng, Lei;Tong, Yiping;Li, Wenjing;Hu, Mengyun;Zhang, Yingjun
关键词：nitrate-starvation response; nitrogen use efficiency; primary nitrate response; protein interaction; transcription factor; wheat
-
Nullification of GFTs fortifies bioactive folates in foxtail millet
作者：Pang, Jianzhou;Diao, Xianmin;Pang, Jianzhou;Zhang, Wei;Zhang, Yanyan;Zhang, Shihui;Wang, Yannan;Zhi, Hui;Diao, Xianmin;Zhang, Chunyi;Liang, Qiuju
关键词：
-
Effect of high-molecular-weight glutenin subunits silencing on dough aggregation characteristics
作者：Wang, Qi;Wang, Qi;Wang, Zhicheng;Wang, Zehao;Liang, Ying;Zhang, Xia;Wang, Jinshui;Duan, Yaqian;Guo, Haimei;Zhang, Yingjun
关键词：High-molecular-weight glutenin subunit; Aggregation; Secondary structure of dough; Disulfide bond; Dough microstructure
-
Population Genetic Analysis of a Bread Wheat Panel from Northern and Huang-Huai Agro-Ecological Regions in China
作者：Jin, Xiaojie;Song, Zheng;Zhao, Xiaobin;Peng, Yanchun;Jin, Xiaojie;Liu, Qian;Zhao, Yun;Zhang, Yingjun;Gao, Huimin;He, Wenchuang;Wang, Guijuan;Ren, Xifeng;Zhou, Yu;Ren, Xifeng
关键词：bread wheat; SNP; population structure; genetic diversity; ancestry informative markers
-
Targeted metabolome and transcriptome analyses reveal changes in gibberellin and related cell wall-acting enzyme-encoding genes during stipe elongation in Flammulina filiformis
作者：Li, Hui;Yao, Sen;Xia, Weiwei;Ma, Xinbin;Shi, Lei;Ju, Huimin;Li, Ziyan;Zhong, Yingli;Tao, Yongxin;Shi, Lei;Ju, Huimin;Li, Ziyan;Zhong, Yingli;Xie, Baogui;Tao, Yongxin
关键词：Flammulina filiformis; gibberellin; stipe elongation; targeted metabolome; transcriptome
-
Pangenome analysis reveals genomic variations associated with domestication traits in broomcorn millet
作者：Chen, Jinfeng;Liu, Minxuan;He, Qiang;Zhang, Wei;Gao, Yuanzhu;Jia, Guanqing;Lu, Ping;Zhi, Hui;Tang, Sha;Diao, Xianmin;Chen, Jinfeng;Liu, Yang;Guo, Wenlei;Chen, Weiyao;Zhang, Xiaoming;Guo, Wenlei;Chen, Weiyao;Wang, Yongqiang;Liao, Yi;Dong, Kongjun;Ren, Ruiyu;Yang, Tianyu;Zhang, Liyuan;Qi, Mingyu;Li, Zhiguang;Zhao, Min;Wang, Haigang;Wang, Junjie;Qiao, Zhijun;Li, Haiquan;Jiang, Yanmiao;Liu, Guoqing;Song, Xiaoqiang;Deng, Yarui;Li, Hai;Yan, Feng;Dong, Yang;Li, Qingquan;Li, Tao;Yang, Wenyao;Cui, Jianghui;Wang, Hongru;Zhou, Yongfeng
关键词：
-
Combined analysis of metabolome and transcriptome of wheat kernels reveals constitutive defense mechanism against maize weevils
作者：Lv, Liangjie;Guo, Xiaorui;Zhao, Aiju;Liu, Yuping;Li, Hui;Chen, Xiyong
关键词：Triticum aestivum L; Sitophilus zeamais; metabolome; transcriptome; constitutive defense; flavonoids biosynthesis
