Transgenic expression of plastidic glutamine synthetase increases nitrogen uptake and yield in wheat
文献类型： 外文期刊
作者： Hu, Mengyun 1 ; Zhao, Xueqiang 2 ; Liu, Qian 1 ; Hong, Xia 2 ; Zhang, Wei 2 ; Zhang, Yingjun 1 ; Sun, Lijing 1 ; Li, Hui; 1 ;
作者机构： 1.Hebei Acad Agr & Forestry Sci, Inst Cereal & Oil Crops, Hebei Lab Crop Genet & Breeding, Shijiazhuang, Hebei, Peoples R China
2.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing, Peoples R China
3.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab P
关键词： wheat; glutamine synthetase; nitrogen use efficiency
期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：9.803； 五年影响因子：9.555 ）
ISSN： 1467-7644
年卷期： 2018 年 16 卷 11 期
页码：
收录情况： SCI
摘要： The plastidic glutamine synthetase isoform (GS2) plays a key role in nitrogen (N) assimilation. We introduced TaGS2- 2Abpro::TaGS2-2Ab, the favourable allele of TaGS2-2A in the winter wheat (Triticum aestivum) variety Ji5265. Transgenic expression of TaGS2-2Ab significantly increased GS2 abundance and GS activity in leaves. Two consecutive field experiments showed the transgenic lines had higher grain yield, spike number, grain number per spike and 1000-grain weight than did the wild type under both low N and high N conditions. Analysis of N use-related traits showed that transgenic expression of TaGS2-2Ab increased root ability to acquire N, N uptake before and after flowering, remobilization of N to grains and N harvest index. Measurement of chlorophyll content and net photosynthesis rate in flag leaves during grain filling stage revealed that the transgenic lines had prolonged leaf functional duration as compared with the wild type. These results suggest that TaGS2 plays important role in N use, and the favourable allele TaGS2-2Ab is valuable in engineering wheat with improved N use efficiency and grain yield.
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