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Development of a TaqMan-based real-time PCR for detecting duck adenovirus 3

文献类型： 外文期刊

作者： Wan, Chunhe 1 ; Chen, Cuiteng 1 ; Cheng, Longfei 1 ; Fu, Guanghua 1 ; Shi, Shaohua 1 ; Liu, Rongchang 2 ; Chen, Hongme 1 ;

作者机构： 1.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fujian Anim Dis Control Technol Dev Ctr, Fujian Prov Key Lab Avian Dis Control & Prevent, Fuzhou 350013, Fujian, Peoples R China

2.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fujian Anim Dis Control Technol Dev Ctr, Fujian Prov Key Lab Avian Dis Control & Prevent, Fu

关键词： DAdV-3; Detection; TaqMan-based; Real-time PCR assay

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2018 年 261 卷

页码：

收录情况： SCI

摘要： Recently, a novel duck adenovirus (designated as duck adenovirus 3, DAdV-3) was discovered in Muscovy ducks, China. Here, we developed a TaqMan-based real-time PCR assay (qPCR) for the detection of DAdV-3 infection. After the optimization of the qPCR conditions, the lower limit of detection for DAdV-3 infection was 40.9 copies/mu l. No cross-reactivity was observed with other duck-derived pathogens. Intra- and inter-assay variability was less than 2.30%. DAdV-3 was detected in embryos and newly hatched ducklings by qPCR assay, the findings provided evidence of possible vertical transmission of DAdV-3. The developed qPCR analysis showed high specificity, sensitivity, and reproducibility, thereby indicating that it can be used in future investigations on the pathogenesis and epidemiology of DAdV-3.

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