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System for the heterologous expression of NS1 protein of H9N2 avian influenza virus in the ciliate Tetrahymena thermophila

文献类型： 外文期刊

作者： Zhu, Chunhua 1 ; Liu, Xiaodong 2 ; Chi, Hongshu 2 ; Chen, Cuiteng 1 ; Chen, Zhen 1 ; Fu, Guanghua 1 ; Gong, Hui 2 ; Huang 1 ;

作者机构： 1.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350013, Fujian, Peoples R China

2.Fujian Acad Agr Sci, Inst Biotechnol, Fuzhou 350003, Fujian, Peoples R China

3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

4.Fujian Prov Key Lab Avian Dis Control & Prevent, Fuzhou 350013, Fujian, Peoples R China

关键词： avian influenza; heterologous expression; NS1 gene; Tetrahymena; virus

期刊名称：JOURNAL OF VETERINARY MEDICAL SCIENCE （ 影响因子：1.267； 五年影响因子：1.348 ）

ISSN： 0916-7250

年卷期： 2018 年 80 卷 10 期

页码：

收录情况： SCI

摘要： Tetrahymena is commonly used as an alternative eukaryotic system for efficiently expressing heterologous genes. In this study, we inserted the non-structural (NS) 1 gene of avian influenza virus (AIV) into the shuttle vector pD5H8 and transformed conjugating T. thermophila with the recombinant plasmid pD5H8-NS1 by particle bombardment. Positive transformants were selected with paromomycin. We demonstrated that the NS1 protein could be expressed steadily following induction with cadmium in this Tetrahymena system. An enzyme-linked immunosorbent assay detection method was preliminary established using the expressed protein as coating antigens for serodiagnosis. This is the first study in which a Tetrahymena expression system was employed for the expression of the AIV NS1 protein, and it provides a good basis for the development of differential diagnostic kits and vaccines for the prevention and control of avian influenza.

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