System for the heterologous expression of NS1 protein of H9N2 avian influenza virus in the ciliate Tetrahymena thermophila
文献类型： 外文期刊
作者： Zhu, Chunhua 1 ; Liu, Xiaodong 2 ; Chi, Hongshu 2 ; Chen, Cuiteng 1 ; Chen, Zhen 1 ; Fu, Guanghua 1 ; Gong, Hui 2 ; Huang 1 ;
作者机构： 1.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350013, Fujian, Peoples R China
2.Fujian Acad Agr Sci, Inst Biotechnol, Fuzhou 350003, Fujian, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Fujian Prov Key Lab Avian Dis Control & Prevent, Fuzhou 350013, Fujian, Peoples R China
关键词： avian influenza; heterologous expression; NS1 gene; Tetrahymena; virus
期刊名称：JOURNAL OF VETERINARY MEDICAL SCIENCE （ 影响因子：1.267； 五年影响因子：1.348 ）
ISSN： 0916-7250
年卷期： 2018 年 80 卷 10 期
页码：
收录情况： SCI
摘要： Tetrahymena is commonly used as an alternative eukaryotic system for efficiently expressing heterologous genes. In this study, we inserted the non-structural (NS) 1 gene of avian influenza virus (AIV) into the shuttle vector pD5H8 and transformed conjugating T. thermophila with the recombinant plasmid pD5H8-NS1 by particle bombardment. Positive transformants were selected with paromomycin. We demonstrated that the NS1 protein could be expressed steadily following induction with cadmium in this Tetrahymena system. An enzyme-linked immunosorbent assay detection method was preliminary established using the expressed protein as coating antigens for serodiagnosis. This is the first study in which a Tetrahymena expression system was employed for the expression of the AIV NS1 protein, and it provides a good basis for the development of differential diagnostic kits and vaccines for the prevention and control of avian influenza.
- 相关文献
作者其他论文 更多>>
-
Specific detection of pigeon parvovirus with TaqMan real-time PCR technology
作者：Chen, Cuiteng;Zhu, Chunhua;Chen, Shuyu;Chen, Zhen;Fu, Huanru;Chen, Yuyi;Zhang, Mengyan;Zhang, Wenyu;Huang, Yu;Cheng, Longfei;Wan, Chunhe;Chen, Shuyu;Fu, Huanru;Zhang, Wenyu;Chen, Yuyi;Zhang, Mengyan
关键词：Pigeon parvovirus (PiPV); NS; TaqMan-PCR; Epidemiological investigation
-
Application of selection Index for enhancing resistance to Cryptocarya irritans and Vibrio alginolyticus in large yellow croaker
作者：Wang, Jiaying;Zhao, Ji;Jiang, Pengxing;Xiao, Junzu;Jiang, Zhou;Miao, Lingwei;He, Qian;Zeng, Junjia;Ke, Qiaozhen;Zhou, Tao;Xu, Peng;Wang, Jiaying;Zhao, Ji;Jiang, Pengxing;Xiao, Junzu;Jiang, Zhou;Miao, Lingwei;He, Qian;Zeng, Junjia;Ke, Qiaozhen;Zhou, Tao;Xu, Peng;Liu, Bo;Chi, Hongshu
关键词：selection index; disease resistance trait; genomic selection; large yellow croaker; aquaculture
-
Influence of fermented feed on lipid composition and volatile flavor profile of duck eggs: Insights from multi-omics analysis
作者：Dong, Qishan;Lu, Lizhi;Gu, Tiantian;Tian, Yong;Zong, Yibo;Zeng, Tao;Dong, Qishan;He, Jun;Lu, Lizhi;Gu, Tiantian;Tian, Yong;Zong, Yibo;Zeng, Tao;Sun, Jing;Huang, Yu;Fu, Qiuling
关键词：Volatile flavor; Duck eggs; Lipidomics; Fermented feed; Off-odor
-
Characterization of a Capsule-Deficient Pasteurella multocida Isolated from Cygnus melancoryphus: Genomic, Phenotypic, and Virulence Insights
作者：Jiang, Nansong;Chen, Hongmei;Wang, Weiwei;Liang, Qizhang;Fu, Qiuling;Liu, Rongchang;Fu, Guanghua;Wan, Chunhe;Huang, Yu;Cheng, Longfei;Jiang, Nansong;Chen, Hongmei;Wang, Weiwei;Liang, Qizhang;Fu, Qiuling;Liu, Rongchang;Fu, Guanghua;Wan, Chunhe;Huang, Yu;Cheng, Longfei;Jiang, Nansong;Chen, Hongmei;Wang, Weiwei;Liang, Qizhang;Fu, Qiuling;Liu, Rongchang;Fu, Guanghua;Wan, Chunhe;Huang, Yu;Cheng, Longfei
关键词：
Pasteurella multocida ;Cygnus melancoryphus ; capsule deficiency; FCF147 strain; adaptation; virulence -
Deltacoronavirus HKU11, HKU13, PDCoV (HKU15) and HKU17 spike pseudoviruses enter avian DF-1 cells via clathrin-mediated endocytosis in a Rab5-, Rab7-and pH-dependent manner
作者：Liang, Qi-Zhang;Chen, Wei;Huang, Yu;Ji, Chun-Miao;Wang, Bin;Huang, Yao-Wei;Ji, Chun-Miao;Cong, Feng;Wang, Bin;Huang, Yao-Wei;Huang, Yao-Wei
关键词：Avian deltacoronavirus; porcine deltacoronavirus; entry pathway; pseudovirus; endocytosis
-
Detecting Deleterious Recessive Variants in Large Yellow Croaker Using Data from 7337 Individuals
作者：Jiang, Zhou;Zhao, Ji;Li, Ning;Wang, Jiaying;Pu, Fei;Xu, Peng;Zhou, Tao;Jiang, Zhou;Zhao, Ji;Li, Ning;Wang, Jiaying;Pu, Fei;Xu, Peng;Zhou, Tao;Zhao, Ji;Chi, Hongshu
关键词：Recessive alleles; Lethal mutations; Large yellow croaker; Homozygosity
-
Metformin Enhances Doxycycline Efficacy Against Pasteurella multocida: Evidence from In Vitro, In Vivo, and Morphological Studies
作者：Jiang, Nansong;Wang, Weiwei;Liang, Qizhang;Fu, Qiuling;Liu, Rongchang;Fu, Guanghua;Wan, Chunhe;Cheng, Longfei;Huang, Yu;Chen, Hongmei;Jiang, Nansong;Wang, Weiwei;Liang, Qizhang;Fu, Qiuling;Liu, Rongchang;Fu, Guanghua;Wan, Chunhe;Cheng, Longfei;Huang, Yu;Chen, Hongmei;Jiang, Nansong;Wang, Weiwei;Liang, Qizhang;Fu, Qiuling;Liu, Rongchang;Fu, Guanghua;Wan, Chunhe;Cheng, Longfei;Huang, Yu;Chen, Hongmei
关键词：
Pasteurella multocida ; resistance; metformin; doxycycline; synergistic antibacterial activity
