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A naturally occurring insertion in the RsFLC2 gene associated with late-bolting trait in radish (Raphanus sativus L.)

文献类型： 外文期刊

作者： Wang, Qingbiao 1 ; Zhang, Yongjie 1 ; Zhang, Li 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China

2.Minist Agr, Key Lab Biol & Genet Improvement Hort Crops North, Beijing 100097, Peoples R China

3.Beijing Key Lab Vegetable Germplasm Improvement, Beijing 100097, Peoples R China

关键词： Radish; FLC2; Bolting and flowering; Intron variation; QTL analysis

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN： 1380-3743

年卷期： 2018 年 38 卷 11 期

页码：

收录情况： SCI

摘要： Premature flowering reduces the yield and quality of the harvested fleshy taproot in radish. However, there has been little molecular marker research on the radish late-bolting trait. In this study, F-2 and F-2:3 populations derived from a cross of Ninengo (late-bolting) and Maer (early-bolting) were analyzed to map late-bolting genes. Five hundred insertion and deletion (InDel) markers were designed according to the whole-genome resequencing data of the two parents. A genetic map was constructed based on the F-2 population, and a late-bolting gene was detected in a 1.1-cM region between the markers InDel520 and InDel535 on chromosome R02 that explained the highest (76.4%) phenotypic variance. RsFLC2 was identified as a candidate gene in this region. Notably, Ninengo contains a 1627-bp insertion near the 5 end of the first intron of RsFLC2. Allelic variation analyses in the F-2 population further validated that RsFLC2was associated with the late-bolting trait in radish. The expression pattern of RsFLC2 was significantly different between Ninengo and Maer during vernalization. Vernalization suppressed RsFLC2 expression, and the 1627-bp insertion in the first intron weakened gene repression in Ninengo plants, resulting in late-bolting. This study lays a foundation for uncovering the molecular mechanism of late-bolting and marker-assisted selection for breeding late-bolting varieties of radish.

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