Development of a Cell-Based High-Throughput Screening Assay to Identify Porcine Host Defense Peptide-Inducing Compounds
文献类型： 外文期刊
作者： Deng, Zhuo 1 ; Wang, Jing 2 ; Lyu, Wentao 1 ; Wieneke, Xuwen 1 ; Matts, Robert 3 ; Ma, Xi 4 ; Zhang, Guolong 1 ;
作者机构： 1.Oklahoma State Univ, Dept Anim & Food Sci, Stillwater, OK 74078 USA
2.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing 100097, Peoples R China
3.Oklahoma State Univ, Dept Biochem & Mol Biol, Stillwater, OK 74078 USA
4.China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China
5.Oklahoma State Univ, Ctr Vet Hlth Sci, Dept Physiol Sci, Stillwater, OK 74078 USA
期刊名称：JOURNAL OF IMMUNOLOGY RESEARCH （ 影响因子：4.818； 五年影响因子：4.849 ）
ISSN： 2314-8861
年卷期： 2018 年
页码：
收录情况： SCI
摘要： Novel alternatives to antibiotics are needed for the swine industry, given increasing restrictions on subtherapeutic use of antibiotics. Augmenting the synthesis of endogenous host defense peptides (HDPs) has emerged as a promising antibiotic-alternative approach to disease control and prevention. To facilitate the identification of HDP inducers for swine use, we developed a stable luciferase reporter cell line, IPEC-J2/PBD3-luc, through permanent integration of a luciferase reporter gene driven by a 1.1 kb porcine beta-defensin 3 (PBD3) gene promoter in porcine IPEC-J2 intestinal epithelial cells. Such a stable reporter cell line was employed in a high-throughput screening of 148 epigenetic compounds and 584 natural products, resulting in the identification of 41 unique hits with a minimum strictly standardized mean difference (SSMD) value of 3.0. Among them, 13 compounds were further confirmed to give at least a 5-fold increase in the luciferase activity in the stable reporter cell line, with 12 being histone deacetylase (HDAC) inhibitors. Eight compounds were subsequently observed to be comparable to sodium butyrate in inducing PBD3 mRNA expression in parental IPEC-J2 cells in the low micromolar range. Six HDAC inhibitors including suberoylanilide hydroxamine (SAHA), HC toxin, apicidin, panobinostat, SB939, and LAQ824 were additionally found to be highly effective HDP inducers in a porcine 3D4/31 macrophage cell line. Besides PBD3, other HDP genes such as PBD2 and cathelicidins (PG1-5) were concentration-dependently induced by those compounds in both IPEC-J2 and 3D4/31 cells. Furthermore, the antibacterial activities of 3D4/31 cells were augmented following 24 h exposure to HDAC inhibitors. In conclusion, a cell-based high-throughput screening assay was developed for the discovery of porcine HDP inducers, and newly identified HDP-inducing compounds may have potential to be developed as alternatives to antibiotics for applications in swine and possibly other animal species.
- 相关文献
作者其他论文 更多>>
-
EasyMetagenome: A user-friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research
作者：Bai, Defeng;Xun, Jiani;Ma, Chuang;Luo, Hao;Yang, Haifei;Hou, Huiyu;Lv, Hujie;Wan, Xiulin;Wang, Yao;Yousuf, Salsabeel;Zeng, Meiyin;Zhang, Tianyuan;Gao, Yunyun;Liu, Yong-Xin;Chen, Tong;Ma, Chuang;Yang, Haifei;Cao, Chen;Cao, Xiaofeng;Cui, Jianzhou;Deng, Yuan-Ping;Deng, Zhaochao;Yu, Hao;Zhang, Chunfang;Dong, Wenxin;Dong, Wenxue;Du, Juan;Fang, Qunkai;Fang, Wei;Fang, Yue;Luan, Yaning;Fu, Fangtian;Fu, Min;Fu, Yi-Tian;Gao, He;Ge, Jingping;Guo, Yuhao;Gong, Qinglong;Lou, Wenbo;Gu, Lunda;Yang, Li;Guo, Peng;Hai, Tang;Liu, Hao;He, Jieqiang;He, Zi-Yang;Huang, Can;Ji, Shuai;Jiang, ChangHai;Jiang, Gui-Lai;Jiang, Lingjuan;Jin, Ling N.;Li, Changchao;Kan, Yuhe;Kang, Da;Kou, Jin;Lam, Ka-Lung;Li, Chong;Li, Fuyi;Li, Liwei;Li, Miao;Li, Xin;Li, Ye;Li, Zheng-Tao;Zhu, Chengshuai;Liang, Jing;Mo, Jiayuan;Lin, Yongxin;Liu, Changzhen;Liu, Danni;Zhang, Jing;Chen, Shifu;Liu, Fengqin;Liu, Jia;Liu, Tianrui;Liu, Tingting;Wang, Xinlong;Liu, Xinyuan;Luo, Yuanyuan;Liu, Yaqun;Liu, Bangyan;Liu, Minghao;Lv, Hujie;Ma, Tengfei;Mai, Zongjiong;Niu, Dongze;Pan, Zhuo;Qi, Heyuan;Shi, Zhanyao;Song, Chunjiao;Sun, Fuxiang;Sun, Yan;Tian, Sihui;Wang, Guoliang;Wang, Hongyang;Wang, Hongyu;Wang, Huanhuan;Wang, Jing;Wang, Jun;Wang, Kang;Wang, Leli;Yao, Xiaofang;Wang, Shao-kun;Xiao, Zufei;Xing, Huichun;Xu, Yifan;Yang, Song;Yan, Shu-yan;Zhang, Yi-Bo;Yang, Yuanming;Lei, Yu;Yuan, Zhengrong;Zhang, Chunge;Zhang, Huimin;Zhang, Na;Zhang, Yupeng;Zhang, Zheng;Zhou, Mingda;Zhou, Yuanping;Zhu, Zhihao;Zhu, Lin;Zhu, Yue;Zou, Hongqin;Zuo, Anna;Dong, Wenxuan;Wen, Tao;Chen, Shifu;Chen, Shifu;Li, Guoliang
关键词：metagenome; microbiome; microbiota; pipeline; visualization
-
Calycosin, a Bioactive Isoflavone, Ameliorates Oxidative Stress and Inflammation in Lipopolysaccharide-Induced Intestinal Cell Damage Model via the Nrf2 and NF-κB Signaling Pathways
作者：Li, Lu;Che, Yuyan;Zhu, Longlong;Liu, Zeqing;Song, Yanchen;Chen, Meixia;Wang, Jing;Li, Lu;Che, Yuyan;Song, Yanchen;Zhu, Longlong;Liu, Zeqing;Wu, Qiong;Chen, Meixia;Wang, Jing
关键词：calycosin; cell damage; inflammation; LPS; NF-kappa B pathway; Nrf2 pathway; oxidative stress
-
Chromosome-level genome assembly of tetraploid Chinese cherry (Prunus pseudocerasus)
作者：Zhang, Wei;Wang, Xuncheng;Peng, Junbo;Xing, Qikai;Yan, Jiye;Wang, Jing;Duan, Xuwei;Zhang, Xiaoming;Zhang, Kaichun
关键词：
-
New insights into the cold tolerance of upland switchgrass by integrating a haplotype-resolved genome and multi-omics analysis
作者：Wu, Bingchao;Luo, Dan;Yan, Haidong;Ma, Xixi;Zhu, Jie;Jia, Jiyuan;Sun, Min;Xie, Zheni;Wang, Xiaoshan;Huang, Linkai;Yue, Yuesen;He, Min;Zhao, Bingyu;Xu, Bin;Wang, Jing;Sun, Min
关键词：Upland switchgrass; Haplotype-resolved genomes; Allele-specific expression genes; Cold tolerance
-
Identification and characteristic analysis of PavUGT48 as a novel UDP-glycosyltransferase with dual functions on anthocyanin and amygdalin biosynthesis in sweet cherry
作者：Feng, Chen;Guo, Qingqing;Wu, Chuanbao;Wang, Jing;Zhang, Xiaoming;Yan, Guohua;Zhou, Yu;Wang, Wei;Zhang, Kaichun;Duan, Xuwei;Feng, Chen;Wu, Chuanbao;Wang, Jing;Zhang, Xiaoming;Yan, Guohua;Zhou, Yu;Wang, Wei;Zhang, Kaichun;Duan, Xuwei;Feng, Chen;Wu, Chuanbao;Wang, Jing;Zhang, Xiaoming;Yan, Guohua;Zhou, Yu;Wang, Wei;Zhang, Kaichun;Duan, Xuwei;Feng, Chen;Wu, Chuanbao;Wang, Jing;Zhang, Xiaoming;Yan, Guohua;Zhou, Yu;Wang, Wei;Zhang, Kaichun;Duan, Xuwei;Guo, Qingqing;Xue, Zheyong
关键词：Sweet cherry; UDP-glycosyltransferase; PavUGT48; Anthocyanin; Amygdalin
-
Effects of different rootstocks on fruit quality and non-volatile flavor-related compounds of sweet cherry 'summit'
作者：Wang, Naiyu;Zhang, Xiaoming;Guo, Qingqing;Yan, Guohua;Wang, Jing;Wu, Chuanbao;Zhou, Yu;Zhou, Junman;Zhang, Kaichun;Duan, Xuwei;Wang, Naiyu;Guo, Qingqing;Li, Tianzhong;Wang, Naiyu;Zhang, Xiaoming;Guo, Qingqing;Yan, Guohua;Wang, Jing;Wu, Chuanbao;Zhou, Yu;Zhou, Junman;Zhang, Kaichun;Duan, Xuwei;Wang, Naiyu;Zhang, Xiaoming;Guo, Qingqing;Yan, Guohua;Wang, Jing;Wu, Chuanbao;Zhou, Yu;Zhou, Junman;Zhang, Kaichun;Duan, Xuwei;Zhou, Junman
关键词：Prunus avium; Fertilization; Grafting; Sugar; Organic acid; Phenolic acid
-
Population sequencing of cherry accessions unravels the evolution of Cerasus species and the selection of genetic characteristics in edible cherries (vol 5, 6, 2025)
作者：Lei, Yahui;Jiu, Songtao;Xu, Yan;Lv, Zhengxin;Liu, Xunju;Wang, Lei;Wang, Li;Wang, Jiyuan;Zhang, Zhuo;Wang, Shiping;Zhang, Caixi;Lei, Yahui;Dong, Xiao;Dong, Yang;Chen, Baozheng;Dong, Yang;Bernard, Anthony;Barreneche, Teresa;Elisabeth, Dirlewanger;Cai, Yuliang;Zheng, Wei;Zhang, Xu;Li, Fangdong;Li, Hongwen;Liu, Congli;Li, Ming;Wang, Jing;Zhu, Jijun;Peng, Lei;Yu, Fei;Duan, Shengchang
关键词：
