文献类型: 外文期刊
作者: Zhao, Shuang 1 ; Zhang, Xuemei 1 ; Su, Yanping 4 ; Chen, Yilan 5 ; Liu, Yang 1 ; Sun, Meng 1 ; Qi, Guohui 1 ;
作者机构: 1.Agr Univ Hebei, Dept Forestry, Baoding 071000, Peoples R China
2.Hebei Acad Agr & Forestry Sci, Cotton Res Inst, Shijiazhuang 050000, Hebei, Peoples R China
3.Res Ctr Walnut Engn & Technol Hebei, Lincheng 054304, Peoples R China
4.Normal Univ Langfang, Dept Life Sci, Langfang 065000, Peoples R China
5.Baoding Forest Seedling Management Stn, Baoding 071000, Peoples R China
期刊名称:INTERNATIONAL JOURNAL OF GENOMICS ( 影响因子:2.326; 五年影响因子:2.557 )
ISSN: 2314-436X
年卷期: 2018 年
页码:
收录情况: SCI
摘要: Walnut (Juglans regia L.) is an important woody oilseed species cultivated throughout the world. In this study, comparative transcript profiling was performed using high-throughput RNA sequencing technology at the following three stages of walnut fat synthesis in the "Lvling" walnut cultivar: the initial developmental stage (L1), the fast developing stage (L2), and the last developing stage (L3). A total of 68.18 GB of data were obtained on the three developmental stages, and 92% to 94% of clean data were able to be located to the reference genome. Further comparisons of the transcripts in the three libraries revealed that 724, 2027, and 4817 genes were differentially expressed between the L2 and L1 (L2vsL1), L3 and L2 (L3vsL2), and L3 and L1 (L3vsL1) samples, respectively. Through the GO gene enrichment analysis, differentially expressed genes (DEGs) in L2vsL1, L3vsL2, and L3vsL1 were enriched into 3, 0, and 2 functional categories, respectively. According to the KEGG enrichment analysis, DEGs in L2vsL1, L3vsL2, and L3vsL1 were annotated into 77, 110, and 3717 taxonomic metabolic pathways in the KEGG database, respectively. Next, we analyzed expression levels of genes related to fat synthesis. Our results indicated that ACCase, LACS, and FAD7 were the key genes related to fat synthesis. The high-throughput transcriptome sequencing of walnut in different developmental stages has greatly enriched the current genomic available resources. The comparison of DEGs under different developmental stages identified a wealth of candidate genes involved in fat synthesis, which will facilitate further genetic improvement and molecular studies of the walnut.
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