文献类型： 外文期刊

作者： Ma, Bin ¹ ; Yin, Changfu ² ; Hu, Dailun ² ; Newman, Mark ¹ ; Nicholls, Philip K. ¹ ; Wu, Zhanjun ³ ; Greene, Wayne K.; ¹ ;

作者机构： 1.Murdoch Univ, Sch Vet & Life Sci, Murdoch, WA, Australia

2.Hebei Med Univ, Clin Coll, 309 Jianhuanan St, Shijiazhuang 050031, Hebei, Peoples R China

3.Hebei Acad Agr & Forestry Sci, Inst Cereal & Oil Crop, Shijiazhuang, Hebei, Peoples R China

关键词： Non-myelinating Schwann cells; lymphocyte; dendritic cell; spleen; immunofluorescence staining

期刊名称：EUROPEAN JOURNAL OF HISTOCHEMISTRY （ 影响因子：3.188； 五年影响因子：2.589 ）

ISSN： 1121-760X

年卷期： 2018 年 62 卷 2 期

页码：

收录情况： SCI

摘要： The nervous system and the immune system communicate extensively with each other in order to maintain homeostasis and to regulate the immune response. The peripheral nervous system (PNS) communicates specifically with the immune system according to local interactions, including the "hardwiring" of sympathetic/parasympathetic (efferent) and sensory nerves (afferent) to lymphoid tissue and organs. To reveal this type of bidirectional neuroimmune interaction at the microscopic level, we used immunofluorescent staining of glial fibrillary acidic protein (GFAP) coupled with confocal microscopy/3D reconstruction to reveal the distribution of non-myelinating Schwann cells (NMSCs) and their interactions with immune cells inside mouse spleen. Our results demonstrate i) the presence of an extensive network of NMSC processes in all splenic compartments including the splenic nodules, periarteriolar lymphoid sheath (PALS), marginal zone, trabecula, and red pulp; ii) the close association of NMSC processes with blood vessels (including central arteries and their branches, marginal sinuses, penicillar arterioles and splenic sinuses); iii) the close "synapse-like" interaction/association of NMSC processes with various subsets of dendritic cells (DCs; e.g., CD4(+)CD11c(+) DCs, B220(+)CD11c(+) DCs, and F4/80(+) CD11c(+) DCs), macrophages (F4/80(+)), and lymphocytes (B cells, CD4(+) T helper cells). Our novel findings concerning the distribution of NMSCs and NMSC-leukocytes interactions inside mouse spleen should improve our understanding of the mechanisms through which the PNS affects cellu-lar-and humoral-mediated immune responses in a variety of health conditions and infectious/non-infectious diseases.