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Development of tightly linked markers and identification of candidate genes for Fusarium crown rot resistance in barley by exploiting a near-isogenic line-derived population

文献类型: 外文期刊

作者: Jiang, Yunfeng 1 ; Habib, Ahsan 1 ; Zheng, Zhi 1 ; Zhou, Meixue 3 ; Wei, Yuming 2 ; Zheng, You-Liang 2 ; Liu, Chunji 1 ;

作者机构: 1.Commonwealth Sci & Ind Res Org, Agr & Food, St Lucia, Qld 4067, Australia

2.Sichuan Agr Univ, Triticeae Res Inst, Chengdu 611130, Sichuan, Peoples R China

3.Univ Tasmania, Sch Land & Food, Private Bag 54, Hobart, Tas 7001, Australia

4.Univ Tasmania, Tasmanian Inst Agr, Private Bag 54, Hobart, Tas 7001, Australia

5.Khulna Univ, Biotechnol & Genet Engn Discipline, Khulna 9208, Bangladesh

6.Hebei Acad Agr & Forestry Sci, Natl Foxtail Millet Improvement Ctr, Inst Millet Crops, Shijiazhu

期刊名称:THEORETICAL AND APPLIED GENETICS ( 影响因子:5.699; 五年影响因子:5.565 )

ISSN: 0040-5752

年卷期: 2019 年 132 卷 1 期

页码:

收录情况: SCI

摘要: Key messageThis study demonstrates the feasibility of developing co-segregating markers and identifying candidate genes for Fusarium crown rot resistance in barley based on the generation and exploitation of a near-isogenic line-derived large population.AbstractFusarium crown rot (FCR) is a chronic and severe disease in cereals in semi-arid regions worldwide. Previous studies showed that FCR assessment could be affected by many factors including plant height, growth rate as well as drought stress. Thus, accurate assessment, which is essential for detailed mapping of any locus conferring FCR resistance, is difficult. Targeting one of the resistance loci reported earlier, we developed a near-isogenic line-derived population consisting of 1820 F9 lines. By analysing this population, the Qcrs.cpi-4H locus was mapped to an interval of 0.09cM covering a physical distance of about 637kb and 13 markers co-segregating with the targeted locus were developed. Candidate genes underlying the resistance locus were identified by analysing the expression and sequence variation of genes in the targeted interval. The accurate localization and the development of co-segregating markers should facilitate the incorporation of this large-effect QTL into breeding programmes as well as the cloning of gene(s) underlying the locus.

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