A modified protocol for total RNA isolation from different oil palm (Elaeis guineensis) tissues using cetyltrimethylammonium bromide
文献类型： 外文期刊
作者： Qadri, Rashad 1 ; Iqbal, Amjad 1 ; Wu, Yi 1 ; Li, Jing 1 ; Nisar, Numrah 4 ; Azam, Muhammad 2 ; Yang, Yaodong 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Hainan Key Lab Trop Oil Crops Biol, Coconut Res Inst, Wenchang 571339, Hainan, Peoples R China
2.Univ Agr Faisalabad, Inst Hort Sci, Faisalabad, Pakistan
3.Abdul Wali Khan Univ Mardan, Dept Agr, Mardan, Pakistan
4.Women Univ Lahore, Lahore Coll, Dept Environm Sci, Lahore 54600, Pakistan
关键词： CTAB method; gene expression; oil palm; RNA extraction
期刊名称：CURRENT SCIENCE （ 影响因子：1.102； 五年影响因子：1.163 ）
ISSN： 0011-3891
年卷期： 2019 年 116 卷 3 期
页码：
收录情况： SCI
摘要： Extraction of high-quality RNA from oil palm tissues is challenging due to the presence of polysaccharides, polyphenols and other complexes that co-precipitate with RNA. Therefore, isolation of high-quality RNA from oil palm is challenging due to the presence of varying amounts of these constituents in diverse tissues. This communication describes a modified RNA extraction protocol based on the cetyltrimethylammonium bromide (CTAB) method which is useful for extracting high-quality RNA from different oil palm tissues. Total RNA isolation using a modified CTAB protocol was compared with two different methods, a conventional TRIzol method and the method for RNA isolation from palms (MRIP). Both methods were useful for isolating RNA from leaf tissues; however, they were not effective in isolating RNA from other tissues. The current protocol based on a modified CTAB method was efficient in isolating high-quality total RNA from oil palm fruit tissues, including mesocarp and endosperm, stem, root and flower tissues. This modified CTAB-based protocol gave approximately 20-30 mu g of total RNA from 150 mg of tissue within 5-6 h.
- 相关文献
作者其他论文 更多>>
-
Research Progress on Gene Regulation of Plant Floral Organogenesis
作者：Zhou, Lixia;Yang, Yaodong;Zhou, Lixia;Iqbal, Amjad;Yang, Yaodong;Iqbal, Amjad;Yang, Mengdi
关键词：floral organ; gene regulation; MADS box; AP2/ERF; NAP
-
Interaction of R2R3-MYB transcription factor EgMYB111 with ABA receptors enhances cold tolerance in oil palm
作者：Zhou, Lixia;Li, Rui;Yang, Xuanwen;Yang, Yaodong;Zhou, Yongfeng;Zhou, Lixia;Li, Rui;Yang, Yaodong;Iqbal, Amjad;Yang, Xuanwen;Peng, Yanling;Wang, Yiwen;Xu, Qi;Su, Xiangnian;Zhou, Yongfeng;Wang, Yiwen;Iqbal, Amjad
关键词：Elaeis guineensis; Oil palm; MYB transcription factors; EgMYB111; ABA signaling pathway; Cold tolerance
-
Assessment of bisulfite sequencing alignment tools for whole genome analysis in plants
作者：Wu, Qiufei;Yang, Yaodong;Zhou, Lixia;Wu, Qiufei;Yang, Yaodong;Iqbal, Amjad;Zhou, Lixia;Yang, Mengdi;Iqbal, Amjad
关键词：DNA methylation; Whole genome bisulfite sequencing; Sequence alignment; Plant
-
Genome-wide characterization of the NRT1 family members under cold stress in Coconut (Cocos nucifera L.)
作者：Liu, Xiaomei;Li, Jing;Luo, Dan;Ding, Hao;Zhang, Mengluo;Gao, Ping;Mehvish, Ambreen;Sun, Xiwei;Tong, Chaoqun;Wu, Qiufei;Yang, Yaodong;Iqbal, Amjad;Liu, Xiaomei;Li, Jing;Luo, Dan;Ding, Hao;Zhang, Mengluo;Gao, Ping;Mehvish, Ambreen;Sun, Xiwei;Tong, Chaoqun;Wu, Qiufei;Yang, Yaodong
关键词：Nitrate transporters; Coconut; Gene structure; Nitrogen; Cold stress
-
Sensory analysis combined with targeted metabolomics reveal quality changes in solid and liquid endosperm of postharvest tender coconut
作者：Shen, Xiaojun;Li, Jing;Sun, Xiwei;Li, Jia;Li, Dongxia;Yang, Yaodong;Shen, Xiaojun;Li, Jing;Sun, Xiwei;Yang, Yaodong
关键词：Coconut water; Coconut flesh/meat; Sugars; Organic acids; Amino acids; Fatty acids
-
Identification of key genes and signaling pathways in coconut (Cocos nucifera L.) under drought stress via comparative transcriptome analysis
作者：Akter, Md. Babul;Li, Jing;Lv, Xiang;Mehvish, Ambreen;Abu Sayed, Md.;Yang, Yaodong;Akter, Md. Babul;Li, Jing;Lv, Xiang;Saand, Mumtaz Ali;Mehvish, Ambreen;Abu Sayed, Md.;Yang, Yaodong;Saand, Mumtaz Ali
关键词：Drought stress; Antioxidant enzymes; Transcriptome; Plant hormone signal transduction; MAPK signaling pathway; Differentially expressed genes (DEGs) and coconut
-
Genome-wide association analysis reveals regulatory genes for the metabolite synthesis of 2-acetyl-1-pyrroline in aromatic coconut (Cocos nucifera L.)
作者：Ding, Hao;Lv, Xiang;Liu, Xiaomei;Sun, Xiwei;Li, Jing;Yang, Yaodong;Ding, Hao;Lv, Xiang;Zhou, Guangzhen;Liu, Xiaomei;Sun, Xiwei;Li, Jing;Yang, Yaodong;Zhou, Guangzhen;Iqbal, Amjad
关键词：Aromatic coconut; 2AP; SNP; Genome-wide association study (GWAS); Haplotype analysis
