A modified protocol for total RNA isolation from different oil palm (Elaeis guineensis) tissues using cetyltrimethylammonium bromide
文献类型： 外文期刊
作者： Qadri, Rashad 1 ; Iqbal, Amjad 1 ; Wu, Yi 1 ; Li, Jing 1 ; Nisar, Numrah 4 ; Azam, Muhammad 2 ; Yang, Yaodong 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Hainan Key Lab Trop Oil Crops Biol, Coconut Res Inst, Wenchang 571339, Hainan, Peoples R China
2.Univ Agr Faisalabad, Inst Hort Sci, Faisalabad, Pakistan
3.Abdul Wali Khan Univ Mardan, Dept Agr, Mardan, Pakistan
4.Women Univ Lahore, Lahore Coll, Dept Environm Sci, Lahore 54600, Pakistan
关键词： CTAB method; gene expression; oil palm; RNA extraction
期刊名称：CURRENT SCIENCE （ 影响因子：1.102； 五年影响因子：1.163 ）
ISSN： 0011-3891
年卷期： 2019 年 116 卷 3 期
页码：
收录情况： SCI
摘要： Extraction of high-quality RNA from oil palm tissues is challenging due to the presence of polysaccharides, polyphenols and other complexes that co-precipitate with RNA. Therefore, isolation of high-quality RNA from oil palm is challenging due to the presence of varying amounts of these constituents in diverse tissues. This communication describes a modified RNA extraction protocol based on the cetyltrimethylammonium bromide (CTAB) method which is useful for extracting high-quality RNA from different oil palm tissues. Total RNA isolation using a modified CTAB protocol was compared with two different methods, a conventional TRIzol method and the method for RNA isolation from palms (MRIP). Both methods were useful for isolating RNA from leaf tissues; however, they were not effective in isolating RNA from other tissues. The current protocol based on a modified CTAB method was efficient in isolating high-quality total RNA from oil palm fruit tissues, including mesocarp and endosperm, stem, root and flower tissues. This modified CTAB-based protocol gave approximately 20-30 mu g of total RNA from 150 mg of tissue within 5-6 h.
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