Development of Insertion and Deletion Markers for Bottle Gourd Based on Restriction Site-associated DNA Sequencing Data
文献类型： 外文期刊
作者： Wu Xinyi 1 ; Xu Pei 1 ; Wu Xiaohua 1 ; Wang Baogen 1 ; Lu Zhongfu 1 ; Li Guojing 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Vegetables, State Key Lab Breeding Base Sustainable Control P, Hangzhou 310021, Zhejiang, Peoples R China
期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：3.032； ）
ISSN： 2095-9885
年卷期： 2017 年 3 卷 1 期
页码：
收录情况： SCI
摘要： Bottle gourd is an important cucurbit crop worldwide. To provide more available molecular markers for this crop, a bioinformatic approach was employed to develop insertion-deletions (InDels) markers in bottle gourd based on restriction site-associated DNA sequencing (RAD-Seq) data. A total of 892 Indels were predicted, with the length varying from 1 bp to 167 bp. Single-nucleotide InDels were the predominant types of InDels. To validate these InDels, PCR primers were designed from 162 loci where InDels longer than 2 bp were predicated. A total of 112 InDels were found to be polymorphic among 9 bottle gourd accessions under investigation. The rate of prediction accuracy was thus at a high level of 72.7%. DNA fingerprinting for 4 cultivars were performed using 8 selected Indels markers, demonstrating the usefulness of these markers.
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