文献类型： 外文期刊

作者： Luo Chang ¹ ; Chen Dongliang ¹ ; Cheng Xi ¹ ; Liu Hua ¹ ; Li Yahui ¹ ; Huang Conglin ² ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing Engn Res Ctr Funct Floriculture, Beijing Key Lab Agr Genet Resources & Biotechnol, Beijing 100097, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing Engn R

期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：3.032； ）

ISSN： 2095-9885

年卷期： 2018 年 4 卷 2 期

页码：

收录情况： SCI

摘要： To further test the accuracy and rationality of the Chinese classification system for chrysanthemum (Chrysanthemum morifolium), 10 simple sequence repeat (SSR) markers were used to identify a collection of 88 chrysanthemum and its related genera accessions. In total, 42 effective alleles across 88 accessions were detected; 3 429 bands were obtained by PCR amplification, including 2 630 polymorphic bands. The similarity coefficient ranged from 0.53 to 0.88. Cluster analysis based on UPGMA illustrated that the wild species and large-flower cultivars were first divided into two clusters, then the large-flower cultivars formed five distinct groups according to petal type, indicated that petal type can be a classification criterion. In the wild species cluster, C. vestitum and C. zawadaskii grouped with A. trilobata, suggested that the Ajania genera was closely related to the Chrysanthemum genera. 'Hangbaiju', 'Gongju' and 'Chuju' were grouped together, and `Boju', O. longilobus and C. mongolicum constituted another branch, showed a correlation with geographic region of origin. Population structure analysis was subsequently performed with K values ranging from 2 to 10, and the most likely estimate for the population structure is five subpopulations, which is nearly consistent with the clustering results. Principle component analysis was further performed to verify the classification results. The results showed that these SSR markers are very powerful for studying genetic relationships and will be useful tools in the identification and classification of chrysanthemum.