Genome-wide characterization of DNA methyltransferase family genes implies GhDMT6 improving tolerance of salt and drought on cotton
文献类型： 外文期刊
作者： Yang, Xiaomin 1 ; Bai, Zhigang 2 ; He, Yunxin 3 ; Wang, Ning 4 ; Sun, Liangqing 2 ; Li, Yongqi 2 ; Yin, Zujun 1 ; Wang, Xiaoge 1 ; Zhang, Binglei 1 ; Han, Mingge 1 ; Lu, Xuke 1 ; Chen, Xiugui 1 ; Wang, Delong 1 ; Wang, Junjuan 1 ; Wang, Shuai 1 ; Guo, Lixue 1 ; Chen, Chao 1 ; Feng, Keyun 4 ; Ye, Wuwei 1 ;
作者机构： 1.Zhengzhou Univ, Chinese Acad Agr Sci Res Base, Sch Agr Sci, State Key Lab Cotton Biobreeding & Integrated Util, Anyang 455000, Henan, Peoples R China
2.Cash Crop Res Inst Jiangxi Prov, Jiujiang 332105, Jiangxi, Peoples R China
3.Hunan Inst Cotton Sci, Changde 415101, Hunan, Peoples R China
4.Gansu Acad Agr Sci, Inst Crop Sci, Lanzhou 730070, Gansu, Peoples R China
关键词： C5-MTase; Gossypium raimondii; Gossypium arboreum; Gossypium hirsutum; Abiotic stress
期刊名称：BMC PLANT BIOLOGY （ 影响因子：5.3； 五年影响因子：5.9 ）
ISSN： 1471-2229
年卷期： 2024 年 24 卷 1 期
页码：
收录情况： SCI
摘要： Background DNA methylation is an important epigenetic mode of genomic DNA modification and plays a vital role in maintaining epigenetic content and regulating gene expression. Cytosine-5 DNA methyltransferase (C5-MTase) are the key enzymes in the process of DNA methylation. However, there is no systematic analysis of the C5-MTase in cotton so far, and the function of DNMT2 genes has not been studied. Methods In this study, the whole genome of cotton C5-MTase coding genes was identified and analyzed using a bioinformatics method based on information from the cotton genome, and the function of GhDMT6 was further validated by VIGS experiments and subcellular localization analysis. Results 33 C5-MTases were identified from three cotton genomes, and were divided into four subfamilies by systematic evolutionary analysis. After the protein domain alignment of C5-MTases in cotton, 6 highly conserved motifs were found in the C-terminus of 33 proteins involved in methylation modification, which indicated that C5-MTases had a basic catalytic methylation function. These proteins were divided into four classes based on the N-terminal difference, of which DNMT2 lacks the N-terminal regulatory domain. The expression of C5-MTases in different parts of cotton was different under different stress treatments, which indicated the functional diversity of cotton C5-MTase gene family. Among the C5-MTases, the GhDMT6 had a obvious up-regulated expression. After silencing GhDMT6 with VIGS, the phenotype of cotton seedlings under different stress treatments showed a significant difference. Compared with cotton seedlings that did not silence GhDMT6, cotton seedlings silencing GhDMT6 showed significant stress resistance. Conclusion The results show that C5-MTases plays an important role in cotton stress response, which is beneficial to further explore the function of DNMT2 subfamily genes.
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