文献类型： 外文期刊

作者： Yang, Xiaomin ¹ ; Bai, Zhigang ² ; He, Yunxin ³ ; Wang, Ning ⁴ ; Sun, Liangqing ² ; Li, Yongqi ² ; Yin, Zujun ¹ ; Wang, Xiaoge ¹ ; Zhang, Binglei ¹ ; Han, Mingge ¹ ; Lu, Xuke ¹ ; Chen, Xiugui ¹ ; Wang, Delong ¹ ; Wang, Junjuan ¹ ; Wang, Shuai ¹ ; Guo, Lixue ¹ ; Chen, Chao ¹ ; Feng, Keyun ⁴ ; Ye, Wuwei ¹ ;

作者机构： 1.Zhengzhou Univ, Chinese Acad Agr Sci Res Base, Sch Agr Sci, State Key Lab Cotton Biobreeding & Integrated Util, Anyang 455000, Henan, Peoples R China

2.Cash Crop Res Inst Jiangxi Prov, Jiujiang 332105, Jiangxi, Peoples R China

3.Hunan Inst Cotton Sci, Changde 415101, Hunan, Peoples R China

4.Gansu Acad Agr Sci, Inst Crop Sci, Lanzhou 730070, Gansu, Peoples R China

关键词： C5-MTase; Gossypium raimondii; Gossypium arboreum; Gossypium hirsutum; Abiotic stress

期刊名称：BMC PLANT BIOLOGY （ 影响因子：5.3； 五年影响因子：5.9 ）

ISSN： 1471-2229

年卷期： 2024 年 24 卷 1 期

页码：

收录情况： SCI

摘要： Background DNA methylation is an important epigenetic mode of genomic DNA modification and plays a vital role in maintaining epigenetic content and regulating gene expression. Cytosine-5 DNA methyltransferase (C5-MTase) are the key enzymes in the process of DNA methylation. However, there is no systematic analysis of the C5-MTase in cotton so far, and the function of DNMT2 genes has not been studied. Methods In this study, the whole genome of cotton C5-MTase coding genes was identified and analyzed using a bioinformatics method based on information from the cotton genome, and the function of GhDMT6 was further validated by VIGS experiments and subcellular localization analysis. Results 33 C5-MTases were identified from three cotton genomes, and were divided into four subfamilies by systematic evolutionary analysis. After the protein domain alignment of C5-MTases in cotton, 6 highly conserved motifs were found in the C-terminus of 33 proteins involved in methylation modification, which indicated that C5-MTases had a basic catalytic methylation function. These proteins were divided into four classes based on the N-terminal difference, of which DNMT2 lacks the N-terminal regulatory domain. The expression of C5-MTases in different parts of cotton was different under different stress treatments, which indicated the functional diversity of cotton C5-MTase gene family. Among the C5-MTases, the GhDMT6 had a obvious up-regulated expression. After silencing GhDMT6 with VIGS, the phenotype of cotton seedlings under different stress treatments showed a significant difference. Compared with cotton seedlings that did not silence GhDMT6, cotton seedlings silencing GhDMT6 showed significant stress resistance. Conclusion The results show that C5-MTases plays an important role in cotton stress response, which is beneficial to further explore the function of DNMT2 subfamily genes.