Functional analysis of MeCIPK23 and MeCBL1/9 in cassava defense response against Xanthomonas axonopodis pv. manihotis
文献类型： 外文期刊
作者： Yan, Yu 1 ; He, Xinyi 1 ; Hu, Wei 3 ; Liu, Guoyin 1 ; Wang, Peng 1 ; He, Chaozu 1 ; Shi, Haitao 1 ;
作者机构： 1.Hainan Univ, Hainan Key Lab Sustainable Utilizat Trop Bioresou, Haikou 570228, Hainan, Peoples R China
2.Hainan Univ, Coll Biol, Inst Trop Agr & Forestry, Haikou 570228, Hainan, Peoples R China
3.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Biol & Genet Resources Trop Crops, Xueyuan Rd 4, Haikou 571101, Hainan, Peoples R China
关键词： Calcium; CBL-interacting protein kinase (CIPK); Cassava (Manihot esculenta); Cassava bacterial blight; Xanthomonas axonopodis pv. manihotis (Xam)
期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）
ISSN： 0721-7714
年卷期： 2018 年 37 卷 6 期
页码：
收录情况： SCI
摘要： Cassava (Manihot esculenta) is an important food crop in tropical area, but its production is largely affected by cassava bacterial blight. However, the information of defense-related genes in cassava is very limited. Calcium ions play essential roles in plant development and stress signaling pathways. Calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) are crucial components of calcium signals. In this study, systematic expression profile of 25MeCIPKs in response to Xanthomonas axonopodis pv. manihotis (Xam) infection was examined, by which seven candidate MeCIPKs were chosen for functional investigation. Through transient expression in Nicotiana benthamiana leaves, we found that six MeCIPKs (MeCIPK5, MeCIPK8, MeCIPK12, MeCIPK22, MeCIPK23 and MeCIPK24) conferred improved defense response, via regulating the transcripts of several defense-related genes. Notably, we found that MeCIPK23 interacted with MeCBL1 and MeCBL9, and overexpression of these genes conferred improved defense response. On the contrary, virus-induced gene silencing of either MeCIPK23 or MeCBL1/9 or both genes resulted in disease sensitive in cassava. To our knowledge, this is the first study identifying MeCIPK23 as well as MeCBL1 and MeCBL9 that confer enhanced defense response against Xam.
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