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An ETHYLENE RESPONSE FACTOR-MYB Transcription Complex Regulates Furaneol Biosynthesis by Activating QUINONE OXIDOREDUCTASE Expression in Strawberry

文献类型： 外文期刊

作者： Zhang, Yuanyuan 1 ; Yin, Xueren 1 ; Xiao, Yuwei 1 ; Zhang, Zuying 1 ; Li, Shaojia 1 ; Liu, Xiaofen 1 ; Zhang, Bo 1 ; Yang 1 ;

作者机构： 1.Zhejiang Univ, Coll Agr & Biotechnol, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China

2.Zhejiang Univ, Zhejiang Prov Key Lab Hort Plant Integrat Biol, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China

3.Zhejiang Univ, State Agr Minist Lab Hort Plant Growth Dev & Qual, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China

4.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Zhejiang, Peoples R China

5.Univ Nottingham, Sch Biosci, Div Plant & Crop Sci, Sutton Bonington Campus, Loughborough LE12 5RD, Leics, England

6.Univ Florida, Genet Inst, Plant Innovat Ctr, Hort Sci, Gainesville, FL 32611 USA

期刊名称：PLANT PHYSIOLOGY （ 影响因子：8.34； 五年影响因子：8.972 ）

ISSN： 0032-0889

年卷期： 2018 年 178 卷 1 期

页码：

收录情况： SCI

摘要： 4-Hydroxy-2,5-dimethyl-3(2H)-furanone (HDMF) is a major contributor to the aroma of strawberry (Fragaria x ananassa) fruit, and the last step in its biosynthesis is catalysed by Fragaria x ananassa quinone oxidoreductase (FaQR). Here, an ethylene response factor (FaERF#9) was characterized as a positive regulator of the FaQR promoter. Linear regression analysis indicated that FaERF#9 transcript levels were significantly correlated with both FaQR transcripts and furanone content in different strawberry cultivars. Transient overexpression of FaERF#9 in strawberry fruit significantly increased FaQR expression and furaneol production. Yeast one-hybrid assays, however, indicated that FaERF#9 by itself did not bind to the FaQR promoter. An MYB transcription factor (FaMYB98) identified in yeast one-hybrid screening of the strawberry cDNA library was capable of both binding to the promoter and activating the transcription of FaQR by similar to 5.6-fold. Yeast two-hybrid assay and bimolecular fluorescence complementation confirmed a direct protein-protein interaction between FaERF#9 and FaMYB98, and in combination, they activated the FaQR promoter 14-fold in transactivation assays. These results indicate that an ERF-MYB complex containing FaERF#9 and FaMYB98 activates the FaQR promoter and upregulates HDMF biosynthesis in strawberry.

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