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Combination of label-free quantitative proteomics and transcriptomics reveals intraspecific venom variation between the two strains of Tetrastichus brontispae, a parasitoid of two invasive beetles

文献类型： 外文期刊

作者： Tang, Bao-Zhen 1 ; Meng, E. 1 ; Zhang, Hua-Jian 1 ; Zhang, Xiao-Mei 1 ; Asgari, Sassan 3 ; Lin, Ya-Ping 1 ; Lin, Yun-Y 1 ;

作者机构： 1.Fujian Agr & Forestry Univ, State Key Lab Ecol Pest Control Fujian & Taiwan C, Fuzhou 350002, Fujian, Peoples R China

2.Fujian Agr & Forestry Univ, Dept Plant Protect, Fujian Prov Key Lab Insect Ecol, 15 Shangxiadian Rd, Fuzhou, Fujian, Peoples R China

3.Univ Queensland, Sch Biol Sci, Brisbane, Qld 4067, Australia

4.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikou 571101, Hainan, Peoples R China

关键词： Tetrastichus brontispae; Octodonta nipae; Brontispa longissima; Parallel reaction monitoring (PRM); Label-free; Parasitoid venom

期刊名称：JOURNAL OF PROTEOMICS （ 影响因子：4.044； 五年影响因子：4.02 ）

ISSN： 1874-3919

年卷期： 2019 年 192 卷

页码：

收录情况： SCI

摘要： The venom apparatus is a conserved organ in parasitoids that shows adaptations correlated with life-style diversification. Combining transcriptomics and label-free quantitative proteomics, here we explored the venom apparatus components of the endoparasitoid Tetrastichus brontispae (Eulophidae), and provide a comparison of the venom apparatus proteomes between its two closely related strains, T. brontispae-Octodonta nipae (Tb-On) and T. brontispae-Brontispa longissima (Tb-Bl). Tb-Bl targets the B. longissima pupa as its habitual host. However, Tb-On is an experimental derivative of Tb-Bl, which has been exposed to the O. nipae pupa as host consecutively for over 40 generation. Results showed that approximately 1505 venom proteins were identified in the T. brontispae venom apparatus. The extracts contained novel venom proteins, such as 4-coumarate-CoA ligase 4. A comparative venom proteome analysis revealed that significant quantitative and qualitative differences in venom composition exist between the two strains; although the most abundant venom proteins were shared between them. The differentially produced proteins were mainly enriched in fatty acid biosynthesis and melanotic encapsulation response. Six of these enriched proteins presented increased levels in Tb-On, and this result was validated by parallel reaction monitoring (PRM) analysis. Overall, our data reveal that venom composition can evolve quickly and respond to host selection.

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