Identification of suitable reference genes for miRNA quantitation in bumblebee (Hymenoptera: Apidae) response to reproduction
文献类型： 外文期刊
作者： Dong, Jie 1 ; Li, Jilian 1 ; Huang, Jiaxing 1 ; Wu, Jie 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Apicultural Res, Key Lab Insect Pollinator Biol, Minist Agr, Beijing 100093, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Hangzhou 310021, Zhejiang, Peoples R China
关键词： bumblebee; microRNAs; reference gene; real-time quantification; normalization
期刊名称：APIDOLOGIE （ 影响因子：2.318； 五年影响因子：3.24 ）
ISSN： 0044-8435
年卷期： 2019 年 50 卷 1 期
页码：
收录情况： SCI
摘要： The precise quantification of microRNAs (miRNAs) expression level is a critical factor in mastering its functions. We evaluate the suitability of two common genes and ten miRNAs as normalizers for miRNA quantification in the head and ovary at different reproductive status of bumblebees, Bombus lantschouensis by using four different algorithms and one consensus rank approach. For the head and ovary combination, miR-275 was the best candidate. For different tissues, miR-275 was the most stable candidate in the head, while the candidate for the ovary was miR-277. To test the best candidate accuracy, miR-315 was demonstrated to be downregulated based on miR-275 normalization in ovipositor bumblebees. The miR-275 and miR-277 combination is identified to be the most reliable and suitable reference genes for the head and ovary of bumblebees.
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