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Identification of GROWTH-REGULATING FACTOR transcription factors in lettuce (Lactuca sativa) genome and functional analysis of LsaGRF5 in leaf size regulation

文献类型： 外文期刊

作者： Zhang, Bin 1 ; Tong, Yanan 4 ; Luo, Kangsheng 4 ; Zhai, Zhaodong 5 ; Liu, Xue 1 ; Shi, Zhenying 6 ; Zhang, Dechun 4 ; Li, 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China

2.Beijing Key Lab Vegetable Germplasm Improvement, Beijing 100097, Peoples R China

3.Minist Agr & Rural Affairs PR China, Key Lab Biol & Genet Improvement Hort Crops North, Beijing 100097, Peoples R China

4.China Three Gorges Univ, Biotechnol Res Ctr, Yichang 443002, Peoples R China

5.Shandong Normal Univ, Coll Life Sci, Jinan 250014, Peoples R China

6.Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Shanghai Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China

关键词： Lettuce; GROWTH-REGULATING FACTOR; Leaf development; MicroRNA396; Genome-wide analysis

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2021 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Background GROWTH-REGULATING FACTORs (GRFs), a type of plant-specific transcription factors, play important roles in regulating plant growth and development. Although GRF gene family has been identified in various plant species, a genome-wide analysis of this family in lettuce (Lactuca sativa L.) has not been reported yet. Results Here we identified 15 GRF genes in lettuce and performed comprehensive analysis of them, including chromosomal locations, gene structures, and conserved motifs. Through phylogenic analysis, we divided LsaGRFs into six groups. Transactivation assays and subcellular localization of LsaGRF5 showed that this protein is likely to act as a transcriptional factor in the cell nucleus. Furthermore, transgenic lettuce lines overexpressing LsaGRF5 exhibited larger leaves, while smaller leaves were observed in LsaMIR396a overexpression lines, in which LsaGRF5 was down-regulated. Conclusions These results in lettuce provide insight into the molecular mechanism of GRF gene family in regulating leaf growth and development and foundational information for genetic improvement of the lettuce variations specialized in leaf character.

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