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Characterization of sugar composition in Chinese royal jelly by ion chromatography with pulsed amperometric detection

文献类型： 外文期刊

作者： Zhu, Zuoyi 1 ; Zhang, Yu 1 ; Wang, Junhong 1 ; Li, Xue 1 ; Wang, Wei 1 ; Huang, Zhongping 4 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Qual & Stand Agr Prod, Hangzhou 310021, Zhejiang, Peoples R China

2.State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Zhejiang, Peoples R China

3.Minist Agr, Key Lab Creat Agr, Hangzhou 310021, Zhejiang, Peoples R China

4.Zhejiang Univ Technol, Coll Chem Engn, Hangzhou 310014, Zhejiang, Peoples R China

关键词： Royal jelly; Sugar; Composition; Ion chromatography; Pulsed amperometric detection; Geographical discrimination

期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS （ 影响因子：4.556； 五年影响因子：4.89 ）

ISSN： 0889-1575

年卷期： 2019 年 78 卷

页码：

收录情况： SCI

摘要： An ion chromatography coupled with pulsed amperometric detection (IC-PAD) method was developed for the determination of sugars in royal jelly (RJ). The method enables quantification of 19 sugars with higher sensitivity than HPLC method. Good linearity was observed in the range of 0.02-500 mg L-1 with coefficients of determination (R-2) >= 0.9994. The limits of detection (LODs) and limits of quantification (LOQs) were in the range of 1.0-110 mu g L-1 and 3.7-380 mu g L-1, respectively. The average recoveries ranged from 91.0% to 108.0%. This simple method can be an effective alternative to GC method, which requires complicated purification and derivatization steps. It was used for the analysis of 100 Chinese RJ samples from three different areas and the results of analyzed sugars were found to be in agreement with those reported in literatures. The sugar profile results have suggested that some minor sugars are better markers for discrimination in relation to geographical origin.

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