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The Phosphoproteomic Response of Okra (Abelmoschus esculentus L.) Seedlings to Salt Stress

文献类型： 外文期刊

作者： Yu, Chenliang 1 ; Wu, Qinqfei 2 ; Sun, Chendong 3 ; Tang, Mengling 3 ; Sun, Junwei 4 ; Zhan, Yihua 2 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Agr Equipment, Hangzhou 310021, Zhejiang, Peoples R China

2.Zhejiang Univ, Coll Life Sci, State Key Lab Plant Physiol & Biochem, Hangzhou 310058, Zhejiang, Peoples R China

3.Zhejiang A&F Univ, Sch Agr & Food Sci, Key Lab Qual Improvement Agr Prod Zhejiang Prov, Hangzhou 311300, Zhejiang, Peoples R China

4.China Jiliang Univ, Coll Modern Sci & Technol, Hangzhou 310018, Zhejiang, Peoples R China

关键词： differentially phosphorylated protein; okra; phosphoproteome; salt stress; TMT labeling

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2019 年 20 卷 6 期

页码：

收录情况： SCI

摘要： Soil salinization is a major environmental stresses that seriously threatens land use efficiency and crop yields worldwide. Although the overall response of plants to NaCl has been well studied, the contribution of protein phosphorylation to the detoxification and tolerance of NaCl in okra (Abelmoschus esculentus L.) seedlings is unclear. The molecular bases of okra seedlings' responses to 300 mM NaCl stress are discussed in this study. Using a combination of affinity enrichment, tandem mass tag (TMT) labeling and high-performance liquid chromatography-tandem mass spectrometry analysis, a large-scale phosphoproteome analysis was performed in okra. A total of 4341 phosphorylation sites were identified on 2550 proteins, of which 3453 sites of 2268 proteins provided quantitative information. We found that 91 sites were upregulated and 307 sites were downregulated in the NaCl/control comparison group. Subsequently, we performed a systematic bioinformatics analysis including gene ontology annotation, domain annotation, subcellular localization, and Kyoto Encyclopedia of Genes and Genomes pathway annotation. The latter revealed that the differentially expressed proteins were most strongly associated with 'photosynthesis antenna proteins' and 'RNA degradation'. These differentially expressed proteins probably play important roles in salt stress responses in okra. The results should help to increase our understanding of the molecular mechanisms of plant post-translational modifications in response to salt stress.

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