Cloning and expression pattern of odorant receptor 11 in Asian honeybee drones, Apis cerana (Hymenoptera, Apidae)
文献类型： 外文期刊
作者： Liu, Jun Feng 1 ; Yang, Le 1 ; Li, Mang 1 ; He, Xu Jiang 1 ; Wang, Zi Long 1 ; Zeng, Zhi Jiang 1 ;
作者机构： 1.Jiangxi Agr Univ, Honeybee Res Inst, Nanchang 330045, Jiangxi, Peoples R China
2.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikou 571101, Hainan, Peoples R China
3.Kaihua Cty Anim Husb & Vet Bur, Quzhou 324300, Peoples R China
关键词： Apis cerana; Odorant receptor 11; Drones; Mating flight; Sexual development
期刊名称：JOURNAL OF ASIA-PACIFIC ENTOMOLOGY （ 影响因子：1.303； 五年影响因子：1.427 ）
ISSN： 1226-8615
年卷期： 2019 年 22 卷 1 期
页码：
收录情况： SCI
摘要： Odorant receptors play a crucial role in the special recognition of scent molecules in the honeybee olfaction system. The odorant receptor 11 (AmOR11) in western honeybee drones (Apis mellifera) has been demonstrated to specifically bind to 9-oxo-2-decenoic acid (9-ODA) of queens. However, little is known regarding the functions of OR11 Asian honeybee drones (Apis cerana) in the context of their mating activities. In this study, the odorant receptor 11 gene (AcOr11) from A. cerana was cloned, and its expression profiles were examined during two developmental stages (immature and sexually mature) and different physiological statuses (flying and crawling). The cDNA sequence of AcOrl l was highly similar to that of AmOr1 1, and encoded a membrane-coupled protein of 384 amino acids. The results of qRT-PCR indicated that AcOr1 1 was expressed at higher levels in drone antennae compared to brains, and the expression was significantly up-regulated in sexually mature drone brains compared to immature brains. Interestingly, AcOrll expression in brains of mature flying drones was dramatically higher than those of mature crawling drones. To our knowledge, this study demonstrate a link between AcOrl 1 gene expression in the brain of honeybee drones and behavior associated with sexual maturity and mating flight.
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